Web of Science: 21 cites, Scopus: 22 cites, Google Scholar: cites
Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes
Llach, Anna (Centro de Investigación Cardiovascular)
Espinosa Molina, Cristina (Centro de Investigación Cardiovascular)
Alvarez-Lacalle, Enrique (Universitat Politècnica de Catalunya. Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Benítez, Raúl (Universitat Politècnica de Catalunya. Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial)
Hove-Madsen, Leif (Centro de Investigación Cardiovascular)

Data: 2011
Resum: Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1. 45±0. 03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0. 88 sparks µm−1 min−1 while calcium waves were 8. 5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F0) from 1. 77±0. 17 to 1. 12±0. 18 (p = 0. 002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0. 6±0. 3 min−1 to 8. 1±2. 0 min−1, p = 0. 001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; Versió publicada
Matèria: Calcium release ; Sarcoplasmic reticulum
Publicat a: PloS one, Vol. 6, Issue 8 (August 2011) , p. e23708, ISSN 1932-6203

DOI: 10.1371/journal.pone.0023708
PMID: 21897853


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