Per citar aquest document: http://ddd.uab.cat/record/108878
Real-time PCR improves Helicobacter pylori detection in patients with peptic ulcer bleeding.
Ramírez-Lázaro, María José (Universitat Autònoma de Barcelona. Departament de Medicina)
Lario, Sergio (Universitat Autònoma de Barcelona. Departament de Medicina)
Casalots Casado, Álex (Hospital de Sabadell)
Sanfeliu, Esther (Hospital de Sabadell)
Boix, Loreto (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
García Iglesias, Pilar (Universitat Autònoma de Barcelona. Departament de Medicina)
Sánchez Delgado, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina)
Montserrat, Antònia (Universitat Autònoma de Barcelona. Departament de Medicina)
Bella i Cueto, Maria Rosa (Hospital de Sabadell)
Gallach Montero, Marta (Universitat Autònoma de Barcelona. Departament de Medicina)
Sanfeliu, Isabel (Universitat Autònoma de Barcelona. Departament de Medicina)
Segura, Ferran (Universitat Autònoma de Barcelona. Departament de Medicina)
Calvet Calvo, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina)

Data: 2011
Resum: Background and aims: Histological and rapid urease tests to detect H. pylori in biopsy specimens obtained during peptic ulcer bleeding episodes (PUB) often produce false-negative results. We aimed to examine whether immunohistochemistry and real-time PCR can improve the sensitivity of these biopsies. Patients and Methods: We selected 52 histology-negative formalin-fixed paraffin-embedded biopsy specimens obtained during PUB episodes. Additional tests showed 10 were true negatives and 42 were false negatives. We also selected 17 histology-positive biopsy specimens obtained during PUB to use as controls. We performed immunohistochemistry staining and real-time PCR for 16S rRNA, ureA, and 23S rRNA for H. pylori genes on all specimens. Results: All controls were positive for H. pylori on all PCR assays and immunohistochemical staining. Regarding the 52 initially negative biopsies, all PCR tests were significantly more sensitive than immunohistochemical staining (p<0. 01). Sensitivity and specificity were 55% and 80% for 16S rRNA PCR, 43% and 90% for ureA PCR, 41% and 80% for 23S rRNA PCR, and 7% and 100% for immunohistochemical staining, respectively. Combined analysis of PCR assays for two genes were significantly more sensitive than ureA or 23S rRNA PCR tests alone (p<0. 05) and marginally better than 16S rRNA PCR alone. The best combination was 16S rRNA+ureA, with a sensitivity of 64% and a specificity of 80%. Conclusions: Real-time PCR improves the detection of H. pylori infection in histology-negative formalin-fixed paraffin-embedded biopsy samples obtained during PUB episodes. The low reported prevalence of H. pylori in PUB may be due to the failure of conventional tests to detect infection.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; publishedVersion
Matèria: Úlcera pèptica ; Real-time PCR
Publicat a: PLoS one, Vol. 6, Issue 5 (May 2011) , p. e20009, ISSN 1932-6203

DOI: 10.1371/journal.pone.0020009


7 p, 104.4 KB

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