Per citar aquest document: http://ddd.uab.cat/record/112463
Comparative analysis of the acute response of the trout, O. mykiss, head kidney to in vivo challenge with virulent and attenuated infectious hematopoietic necrosis virus and LPS-induced inflammation
Mackenzie, Simon A. (Universitat Autonoma de Barcelona. Departament de Biologia Cellular, de Fisiologia i d'Immunologia)
Balasch Alemany, Joan Carles (Universitat Autonoma de Barcelona. Departament de Biologia Cellular, de Fisiologia i d'Immunologia)
Novoa, Beatriz (Instituto de Investigaciones Marinas (Vigo, Espanya))
Ribas, Laia (Imperial College Faculty of Medicine. Department of Infectious Disease Epidemiology (Londres, Regne Unit))
Roher Armentia, Nerea (Universitat de Barcelona. Departament de Fisiologia)
Krasnov, Aleksei (Akvaforsk, Institutt for akvakulturforskning (Aas, Noruega))
Figueras, Antonio (Instituto de Investigaciones Marinas (Vigo, Espanya))

Data: 2008
Resum: Background: The response of the trout, O. mykiss, head kidney to bacterial lipopolysaccharide (LPS) or active and attenuated infectious hematopoietic necrosis virus (IHNV and attINHV respectively) intraperitoneal challenge, 24 and 72 hours post-injection, was investigated using a salmonid-specific cDNA microarray. Results: The head kidney response to i. p. LPS-induced inflammation in the first instance displays an initial stress reaction involving suppression of major cellular processes, including immune function, followed by a proliferative hematopoietic-type/biogenesis response 3 days after administration. The viral response at the early stage of infection highlights a suppression of hematopoietic and protein biosynthetic function and a stimulation of immune response. In fish infected with IHNV a loss of cellular function including signal transduction, cell cycle and transcriptional activity 72 hours after infection reflects the tissue-specific pathology of IHNV infection. attIHNV treatment on the other hand shows a similar pattern to native IHNV infection at 24 hours however at 72 hours a divergence from the viral response is seen and replace with a recovery response more similar to that observed for LPS is observed. Conclusion: In conclusion we have been able to identify and characterise by transcriptomic analysis two different types of responses to two distinct immune agents, a virus, IHNV and a bacterial cell wall component, LPS and a 'mixed' response to an attenuated IHNV. This type of analysis will lead to a greater understanding of the physiological response and the development of effective immune responses in salmonid fish to different pathogenic and pro-inflammatory agents.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; publishedVersion
Publicat a: BMC Genomics, Vol. 9, N. 141 (March 2008) , ISSN 1471-2164

DOI: 10.1186/1471-2164-9-141


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