Genome-wide analysis of factors affecting transcription elongation and DNA repair : a new role for PAF and Ccr4-not in transcription-coupled repair
Gaillard, Hélène (Centro Andaluz de Biología Molecular y Medicina Regenerativa)
Tous, Cristina (Centro Andaluz de Biología Molecular y Medicina Regenerativa)
Botet, Javier (Universidad de Sevilla. Departamento de Genética)
González Aguilera, Cristina (Centro Andaluz de Biología Molecular y Medicina Regenerativa)
Quintero, María José (Universidad de Sevilla. Departamento de Genética)
Viladevall Masbernat, Laia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
García Rubio, María L. (Centro Andaluz de Biología Molecular y Medicina Regenerativa)
Rodríguez Gil, Alfonso (Universidad de Sevilla. Departamento de Genética)
Marín Rodríguez, Antonio (Universidad de Sevilla. Departamento de Genética)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Revuelta, José Luis (Instituto de Microbiología Bioquímica (Salamanca, Castella i Lleó))
Chávez, Sebastián (Universidad de Sevilla. Departamento de Genética)
Aguilera, Andrés (Centro Andaluz de Biología Molecular y Medicina Regenerativa)

Data:	2009
Resum:	RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; Versió publicada
Matèria:	Transcription factors ; DNA damage ; Damage mechanics ; Yeast ; Mutation ; Ultraviolet radiation ; DNA repair
Publicat a:	PLoS Genetics, Vol. 5, Issue 2 (February 2009) , p. e1000364, ISSN 1553-7404

DOI: 10.1371/journal.pgen.1000364
PMID: 19197357

15 p, 1.7 MB

El registre apareix a les col·leccions:
Articles > Articles publicats