Per citar aquest document: http://ddd.uab.cat/record/112594
Genome-wide analysis of factors affecting transcription elongation and DNA repair : a new role for PAF and Ccr4-not in transcription-coupled repair
Gaillard, Hélène (Centro Andaluz de Biología Molecular y Medicina Regenerativa (Sevilla, Andalusia))
Tous, Cristina (Centro Andaluz de Biología Molecular y Medicina Regenerativa (Sevilla, Andalusia))
Botet, Javier (Universidad de Sevilla. Departamento de Genética)
González Aguilera, Cristina (Centro Andaluz de Biología Molecular y Medicina Regenerativa (Sevilla, Andalusia))
Quintero, María José (Universidad de Sevilla. Departamento de Genética)
Viladevall Masbernat, Laia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
García Rubio, María L. (Centro Andaluz de Biología Molecular y Medicina Regenerativa (Sevilla, Andalusia))
Rodríguez Gil, Alfonso (Universidad de Sevilla. Departamento de Genética)
Marín, Antonio (Universidad de Sevilla. Departamento de Genética)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Revuelta, José Luis (Instituto de Microbiología Bioquímica (Salamanca, Castella i Lleó))
Chávez, Sebastián (Universidad de Sevilla. Departamento de Genética)
Aguilera, Andrés (Centro Andaluz de Biología Molecular y Medicina Regenerativa (Sevilla, Andalusia))

Data: 2009
Resum: RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; publishedVersion
Matèria: Transcription factors ; DNA damage ; Damage mechanics ; Yeast ; Mutation ; Ultraviolet radiation ; DNA repair
Publicat a: PLoS Genetics, Vol. 5, Issue 2 (February 2009) , p. e1000364, ISSN 1553-7390

DOI: 10.1371/journal.pgen.1000364


15 p, 1.7 MB

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