Protein folding and conformational stress in microbial cells producing recombinant proteins : a host comparative overview
Gasser, Brigitte (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria))
Saloheimo, Markku (VTT Technical Research Centre (Espoo, Finlàndia))
Rinas, Ursula (Helmholtz Center for Infection Research (Braunschweig, Alemanya))
Dragosits, Martin (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria))
Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Giuliani, Maria (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia))
Parrilli, Ermenegilda (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia))
Branduardi, Paola (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia))
Lang, Christine (Technical University Berlin. Institute for Microbiology and Genetics)
Porro, Danilo (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia))
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Tutino, Maria Luisa (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia))
Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria))
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Fecha:	2008
Resumen:	Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; Versió publicada
Publicado en:	Microbial cell factories, Vol. 7, N. 11 (April 2008) , p. 1-18, ISSN 1475-2859

DOI: 10.1186/1475-2859-7-11
PMID: 18394160

18 p, 567.4 KB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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