Per citar aquest document: http://ddd.uab.cat/record/113236
Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy
Tortosa Méndez, Raül (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Castells, Xavier (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Vidal Barba, Enric (Fundació Centre de Recerca en Sanitat Animal)
Costa Riu, Carme (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ruiz de Villa, María del Carmen (Universitat de Barcelona. Facultat de Biologia)
Sánchez, Àlex (Universitat de Barcelona. Facultat de Biologia)
Barceló Vernet, Anna (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Torres, Juan María (Instituto Nacional de Investigaciones Agronómicas (Espanya))
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Data: 2011
Resum: Gene expression analysis has proven to be a very useful tool to gain knowledge of the factors involved in the pathogenesis of diseases, particularly in the initial or preclinical stages. With the aim of finding new data on the events occurring in the Central Nervous System in animals affected with Bovine Spongiform Encephalopathy, a comprehensive genome wide gene expression study was conducted at different time points of the disease on mice genetically modified to model the bovine species brain in terms of cellular prion protein. An accurate analysis of the information generated by microarray technique was the key point to assess the biological relevance of the data obtained in terms of Transmissible Spongiform Encephalopathy pathogenesis. Validation of the microarray technique was achieved by RT-PCR confirming the RNA change and immunohistochemistry techniques that verified that expression changes were translated into variable levels of protein for selected genes. Our study reveals changes in the expression of genes, some of them not previously associated with prion diseases, at early stages of the disease previous to the detection of the pathological prion protein, that might have a role in neuronal degeneration and several transcriptional changes showing an important imbalance in the Central Nervous System homeostasis in advanced stages of the disease. Genes whose expression is altered at early stages of the disease should be considered as possible therapeutic targets and potential disease markers in preclinical diagnostic tool development. Genes non-previously related to prion diseases should be taken into consideration for further investigations.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Publicat a: Veterinary research, Vol. 42, N. 109 (October 2011) , p. 1-14, ISSN 1297-9716

DOI: 10.1186/1297-9716-42-109


14 p, 2.7 MB

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