Per citar aquest document: http://ddd.uab.cat/record/113497
Isolation of cell-free bacterial inclusion bodies
Rodríguez Carmona, Escarlata (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Seras Franzoso, Joaquin (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Data: 2010
Resum: Background: Bacterial inclusion bodies are submicron protein clusters usually found in recombinant bacteria that have been traditionally considered as undesirable products from protein production processes. However, being fully biocompatible, they have been recently characterized as nanoparticulate inert materials useful as scaffolds for tissue engineering, with potentially wider applicability in biomedicine and material sciences. Current protocols for inclusion body isolation from Escherichia coli usually offer between 95 to 99% of protein recovery, what in practical terms, might imply extensive bacterial cell contamination, not compatible with the use of inclusion bodies in biological interfaces. Results: Using an appropriate combination of chemical and mechanical cell disruption methods we have established a convenient procedure for the recovery of bacterial inclusion bodies with undetectable levels of viable cell contamination, below 10-1 cfu/ml, keeping the particulate organization of these aggregates regarding size and protein folding features. Conclusions: The application of the developed protocol allows obtaining bacterial free inclusion bodies suitable for use in mammalian cell cultures and other biological interfaces.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Publicat a: Microbial cell factories, Vol. 9, N. 71 (September 2010) , p. 1-9, ISSN 1475-2859

DOI: 10.1186/1475-2859-9-71


9 p, 1.8 MB

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