Glucose and fructose have sugar-specific effects in both liver and skeletal muscle in vivo : a role for liver fructokinase
Fernández Novell, Josep M..
Ramió-Lluch, Laura
Orozco, Anna
Gómez-Foix, Anna M.
Guinovart, Joan J.
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2014
Resumen:	We examined glucose and fructose effects on serine phosphorylation levels of a range of proteins in rat liver and muscle cells. For this, healthy adult rats were subjected to either oral glucose or fructose loads. A mini-array system was utilized to determine serine phosphorylation levels of liver and skeletal muscle proteins. A glucose oral load of 125mg/100g body weight (G 1/2) did not induce changes in phosphorylated serines of the proteins studied. Loading with 250mg/100g body weight of fructose (Fr), which induced similar glycemia levels as G 1/2, significantly increased serine phosphorylation of liver cyclin D3, PI3 kinase/p85, ERK-2, PTP2 and clusterin. The G 1/2 increased serine levels of the skeletal muscle proteins cyclin H, Cdk2, IRAK, total PKC, PTP1B, c-Raf 1, Ras and the β -subunit of the insulin receptor. The Fr induced a significant increase only in muscle serine phosphorylation of PI3 kinase/p85. The incubation of isolated rat hepatocytes with 10mM glucose for 5 min significantly increased serine phosphorylation of 31 proteins. In contrast, incubation with 10mM fructose produced less intense effects. Incubation with 10mM glucose plus 75μM fructose counteracted the effects of the incubation with glucose alone, except those on Raf-1 and Ras. Less marked effects were detected in cultured muscle cells incubated with 10mM glucose or 10mM glucose plus 75μM fructose. Our results suggest that glucose and fructose act as specific functional modulators through a general mechanism that involves liver-generated signals, like micromolar fructosemia, which would inform peripheral tissues of the presence of either glucose- or fructose-derived metabolites.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Glucosa ; Fructosa
Publicado en:	PloS one, 2014 , ISSN 1932-6203

DOI: 10.1371/journal.pone.0109726
PMID: 25330076

32 p, 160.2 KB

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