Microbial communities from different types of natural wastewater treatment systems : vertical and horizontal flow constructed wetlands and biofilters
Adrados, B. (Universitat Politècnica de Catalunya. Departament d'Òptica i Optometria)
Sánchez Martínez, M. Olga (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Arias, Carlos Alberto (Aarhus universitet. Department of Bioscience)
Bécares Mantecón, Eloy (Universidad de León. Departamento de Ecología)
Garrido, L. 1930- (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Mas Gordi, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Brix, H. (Aarhus universitet. Department of Bioscience)
Morató Farreras, Jordi (Universitat Politècnica de Catalunya)

Date:	2014
Abstract:	The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent.
Note:	This is the author's version of a work that was accepted for publication in Water research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water researc, Vol. 55 (May 2014), p. 304-312. DOI 10.1016/j.watres.2014.02.011
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Aigua ; Abastament ; Microbiologia
Published in:	Water research, Vol. 55 (May 2014) , p. 304-312, ISSN 0043-1354

DOI: 10.1016/j.watres.2014.02.011

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