Web of Science: 2 citations, Scopus: 2 citations, Google Scholar: citations
Efficient amplification of chimeric adenovirus 5/40S vectors carrying the short fiber protein of Ad40 in suspension cell cultures
Miralles, Marta (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Segura, Maria Mercedes (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Puig Ferrer, Meritxell (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Bosch i Merino, Assumpció (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Chillón Rodríguez, Miguel (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))

Date: 2012
Abstract: The human adenovirus 40 (Ad40) is a promising tool for gene therapy of intestinal diseases. Since the production of Ad40 in vitro is extremely inefficient, chimeric Adenovirus 5/40S vectors carrying the Ad40 short fiber on the Ad5 capsid have been developed. However, Ad5/40S productivity is low. We hypothesized that low productivity was a result of inefficient viral entry into producer cells during amplification. To this end, we have developed a production strategy based on using 211B cells (expressing Ad5 fiber) during amplification steps, while Ad5/40S infectivity is further improved by adding polybrene during infections. In addition, the optimal harvesting time was determined by evaluating the Ad5/40S viral cycle. The developed production strategy significantly reduces the number of amplification cycles and duration of the process. Finally, to further facilitate Ad5/40S production, 211B cells were adapted to suspension thus allowing to easily upscale the production process in bioreactors.
Grants: Ministerio de Economía y Competitividad ISCIII-PI081162
Ministerio de Economía y Competitividad ISCIII-PI10-00561
Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-1300
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: Article ; recerca ; Versió publicada
Subject: Vector-borne diseases ; Viral vectors ; Viral tropism ; Cell cultures ; Viral packaging ; Penicillin ; Streptomycin ; Gene therapy
Published in: PloS one, Vol. 7, Issue 7 (July 2012) , p. e42073, ISSN 1932-6203

DOI: 10.1371/journal.pone.0042073
PMID: 22860056


9 p, 588.9 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2015-01-28, last modified 2022-05-26



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