Per citar aquest document: http://ddd.uab.cat/record/132793
Sheltering DNA in self-organizing, protein-only nano-shells as artificial viruses for gene delivery
Unzueta Elorza, Ugutz (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Saccardo, Paolo (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Domingo Espín, Joan (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cedano, J. (Universidad de la República (Uruguai). Laboratorio de Inmunologia)
Cuchillo-Solé, O. (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Garcia i Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Céspedes, María Virtudes (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina)
Corchero Nieto, José Luis (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Daura i Ribera, Xavier (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Mangues, Ramon (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina)
Ferrer Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Data: 2014
Resum: By recruiting functional domains supporting DNA condensation, cell binding, internalization, endosomal escape and nuclear transport, modular single-chain polypeptides can be tailored to associate with cargo DNA for cell-targeted gene therapy. Recently, an emerging architectonic principle at the nanoscale has permitted tagging protein monomers for self-organization as protein-only nanoparticles. We have studied here the accommodation of plasmid DNA into protein nanoparticles assembled with the synergistic assistance of end terminal poly-arginines (R9) and poly-histidines (H6). Data indicate a virus-like organization of the complexes, in which a DNA core is surrounded by a solvent-exposed protein layer. This finding validates end-terminal cationic peptides as pleiotropic tags in protein building blocks for the mimicry of viral architecture in artificial viruses, representing a promising alternative to the conventional use of viruses and virus-like particles for nanomedicine and gene therapy.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Llengua: Anglès
Document: article ; recerca ; submittedVersion
Matèria: Artificial viruses ; Gene therapy ; Nanoparticles ; Protein building blocks ; Self-assembling
Publicat a: Nanomedicine, Vol. 10, issue 3 (april 2014) , p. 535-541, ISSN 1549-9634

DOI: 10.1016/j.nano.2013.11.006


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