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Página principal > Artículos > Artículos publicados > Production of functional inclusion bodies in endotoxin-free Escherichia coli |
Fecha: | 2014 |
Resumen: | Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells. |
Ayudas: | Ministerio de Economía y Competitividad RTA2012-00028-C02-02 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132 |
Derechos: | Tots els drets reservats. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | E. coli ; Recombinant protein ; Inclusion bodies ; Endotoxin ; LPS ; LPS-free |
Publicado en: | Applied microbiology and biotechnology, Vol. 98 issue 22 (Nov. 2014) , p. 9229-9238, ISSN 0175-7598 |
Article-Post-print 18 p, 391.8 KB |
Figures-Post-print 8 p, 520.1 KB |