Per citar aquest document: http://ddd.uab.cat/record/137805
Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
Toledo-Rubio, Verónica (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Platas, Gemma (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Domingo Espín, Joan (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Unzueta Elorza, Ugutz (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Steinkamp, Elke (Bernhard-Nocht-Institut für Tropenmedizin)
Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data: 2010
Resum: Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates.
Nota: Número d'acord de subvenció MINECO/BI02007-61194
Nota: Número d'acord de subvenció MINECO/EUI2008-03610
Nota: Número d'acord de subvenció AGAUR/2009/SGR-108
Drets: Tots els drets reservats.
Llengua: Anglès
Document: article ; recerca ; acceptedVersion
Matèria: Protein aggregation ; Nanoparticles ; Dynamic light scattering ; Proto-aggregates ; Soluble aggregates
Publicat a: Journal of biomolecular screening, Vol. 15, No. 4 (April 2010) , p. 453-457, ISSN 1552-454X

DOI: 10.1177/1087057110363911


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