Differences in photosynthesis and terpene content in leaves and roots of wild-type and transgenic Arabidopsis thaliana plants
Blanch Roure, Josep-Salvador (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Peñuelas, Josep (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Llusià Benet, Joan (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Sardans i Galobart, Jordi (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Owen, Susan M. (Centre de Recerca Ecològica i d'Aplicacions Forestals)

Fecha:	2015
Resumen:	We investigated the hypotheses that two different varieties of Arabidopsis thaliana show differences in physiology and terpene production. The two varieties of A. thaliana used in this study were wildtype (WT) and transgenic line (CoxIVFaNES I) genetically modified to emit nerolidol with linalool/nerolidol synthase (COX). Photosynthetic rate, electron transport rate, fluorescence, leaf volatile terpene contents and root volatile terpene contents were analyzed. For both types, we found coeluting αpinene+βocimene, limonene, and humulene in leaves; and in the roots we found coeluting αpinene+βocimene, sabinene+βpinene, βmyrcene, limonene, and humulene. At the end of the growing cycle, COX plants tended to have lower pools of terpene compounds in their leaves, with 78. 6% lower photosynthesis rates and 30. 8% lower electron transport rates, compared with WT plants at that time. The maximal photochemical efficiency Fv/Fm was also significantly lower (25. 5%) in COX plants, indicating that these varieties were more stressed than WT plants. However, COX plants had higher (239%) root terpene contents compared to WT plants. COX plants appear to favor root production of volatile terpenes rather than leaf production. Thus we conclude that there were significant differences between COX and WT plants in terms of terpenoid pools, stress status and physiology.
Ayudas:	European Commission 610028 Ministerio de Economía y Competitividad CGL2013-48074-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-274
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió sotmesa a revisió
Materia:	Arabidopsis thaliana ; FaNES I ; Leaf terpene contents ; Root terpene contents ; Photosynthesis
Publicado en:	Russian Journal of Plant Physiology, Vol. 62, No. 6, p. 823-829 (2015) , ISSN 1021-4437

DOI: 10.1134/S1021443715060035

Pre-print 20 p, 234.4 KB

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