Per citar aquest document: http://ddd.uab.cat/record/141330
Presence and Function of Dopamine Transporter (DAT) in Stallion Sperm : Dopamine Modulates Sperm Motility and Acrosomal Integrity
Urra, Javier A. (Universidad Austral de Chile. Facultad de Ciencias Veterinarias)
Villaroel-Espíndola, Franz (Universidad Austral de Chile. Facultad de Ciencias)
Covarrubias, Alejandra A. (Universidad Austral de Chile. Facultad de Ciencias)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ramírez-Reveco, Alfredo (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Concha, Ilona I. (Universidad Austral de Chile. Facultad de Ciencias)

Data: 2014
Resum: Dopamine is a catecholamine with multiple physiological functions, playing a key role in nervous system; however its participation in reproductive processes and sperm physiology is controversial. High dopamine concentrations have been reported in different portions of the feminine and masculine reproductive tract, although the role fulfilled by this catecholamine in reproductive physiology is as yet unknown. We have previously shown that dopamine type 2 receptor is functional in boar sperm, suggesting that dopamine acts as a physiological modulator of sperm viability, capacitation and motility. In the present study, using immunodetection methods, we revealed the presence of several proteins important for the dopamine uptake and signalling in mammalian sperm, specifically monoamine transporters as dopamine (DAT), serotonin (SERT) and norepinephrine (NET) transporters in equine sperm. We also demonstrated for the first time in equine sperm a functional dopamine transporter using 4-[4-(Dimethylamino)styryl]-N-methylpyridinium iodide (ASP+ ), as substrate. In addition, we also showed that dopamine (1 mM) treatment in vitro, does not affect sperm viability but decreases total and progressive sperm motility. This effect is reversed by blocking the dopamine transporter with the selective inhibitor vanoxerine (GBR12909) and non-selective inhibitors of dopamine reuptake such as nomifensine and bupropion. The effect of dopamine in sperm physiology was evaluated and we demonstrated that acrosome integrity and thyrosine phosphorylation in equine sperm is significantly reduced at high concentrations of this catecholamine. In summary, our results revealed the presence of monoamine transporter DAT, NET and SERT in equine sperm, and that the dopamine uptake by DAT can regulate sperm function, specifically acrosomal integrity and sperm motility.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; recerca ; publishedVersion
Matèria: Sperm ; Dopamine ; Equines ; Dopamine transporters ; Catecholamines ; Protein extraction ; Reproductive physiology ; Acrosomes
Publicat a: PLoS one, Vol. 9, Issue 11 (November 2014) , p. e112834, ISSN 1932-6203

DOI: 10.1371/journal.pone.0112834


9 p, 1.9 MB

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