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A Reusable Impedimetric Aptasensor for Detection of Thrombin Employing a Graphite-Epoxy Composite Electrode
Ocaña Tejada, Cristina (Universitat Autònoma de Barcelona. Grup Sensors i Biosensors)
Pacios Pujadó, Mercè (Universitat Autònoma de Barcelona. Grup Sensors i Biosensors)
Valle Zafra, Manuel del (Universitat Autònoma de Barcelona. Grup Sensors i Biosensors)

Data: 2012
Resum: Here, we report the application of a label-free electrochemical aptasensor based on a graphite-epoxy composite electrode for the detection of thrombin; in this work, aptamers were immobilized onto the electrodes surface using wet physical adsorption. The detection principle is based on the changes of the interfacial properties of the electrode; these were probed in the presence of the reversible redox couple [Fe(CN)6]3−/[Fe(CN)6]4− using impedance measurements. The electrode surface was partially blocked due to formation of aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by Electrochemical Impedance Spectroscopy (EIS). The aptasensor showed a linear response for thrombin in the range of 7. 5 pM to 75 pM and a detection limit of 4. 5 pM. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2. 0 M NaCl solution at 42 °C, showing its operation for different cycles. The interference response caused by main proteins in serum has been characterized.
Drets: Tots els drets reservats
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Aptamer ; Thrombin ; Electrochemical impedance spectroscopy ; Labeless ; Adsorption
Publicat a: Sensors 2012,, Vol. 12 (2012) , p. 3037-3048, ISSN 1424-8220

12 p, 402.3 KB

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