Per citar aquest document: http://ddd.uab.cat/record/154673
Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes
Ruyra Ripoll, Àngels (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cano Sarabia, Mary (Institut Català de Nanociència i Nanotecnologia)
García-Valtanen, Pablo (Universidad Miguel Hernández. Instituto de Biología Celular y Molecular)
Yero Corona, Daniel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gibert, Isidre (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Mackenzie, Simon A. (University of Stirling. Institute of Aguaculture)
Estepa, Amparo (Universidad Miguel Hernández. Instituto de Biología Celular y Molecular)
Maspoch Comamala, Daniel (Institut Català de Nanociència i Nanotecnologia)
Roher Armentia, Nerea (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Data: 2014
Resum: Herein we report the use of immunostimulant-loaded nanoliposomes (called NLcliposomes) as a strategy to protect fish against bacterial and/or viral infections. This work entailed developing a method for in vivo tracking of the liposomes administered to adult zebrafish that enables evaluation of their in vivo dynamics and characterisation of their tissue distribution. The NLc liposomes, which co-encapsulate poly(I:C) and LPS, accumulate in immune tissues and in immunologically relevant cells such as macrophages, as has been assessed in trout primary cell cultures. They protect zebrafish against otherwise lethal bacterial (Pseudomonas aeruginosa PAO1) and viral (Spring Viraemia of Carp Virus) infections regardless of whether they are administered by injection or by immersion, as demonstrated in a series of in vivo infection experiments with adult zebrafish. Importantly, protection was not achieved in fish that had been treated with empty liposomes or with a mixture of the free immunostimulants. Our findings indicate that stimulation of the innate immune system with co-encapsulated immunostimulants in nano-liposomes is a promising strategy to simultaneously improve the levels of protection against bacterial and viral infections in fish.
Nota: Número d'acord de subvenció MINECO/AGL2012-33877
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Immunostimulant ; Infection model ; Innate immunity ; Liposome ; Macrophageh ; Nanoencapsulation ; Zebrafish
Publicat a: Vaccine, Vol. 32, Issue 31 (June 2014) , p. 3955-3962, ISSN 0264-410X

DOI: 10.1016/j.vaccine.2014.05.010


8 p, 2.0 MB

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