Decoding the genetic and functional diversity of the DSF Quorum-Sensing system in Stenotrophomonas maltophilia
Huedo Moreno, Pol (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Yero, Daniel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martínez-Servat, Sònia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ruyra Ripoll, Àngels (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Roher Armentia, Nerea (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Daura i Ribera, Xavier (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Gibert, Isidre (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2015
Resum:	Stenotrophomonas maltophilia uses the Diffusible Signal Factor (DSF) quorum sensing (QS) system to mediate intra- and inter-specific signaling and regulate virulence-related processes. The components of this system are encoded by the rpf cluster, with genes rpfF and rpfC encoding for the DSF synthase RpfF and sensor RpfC, respectively. Recently, we have shown that there exist two variants of the rpf cluster (rpf-1 and rpf-2), distinguishing two groups of S. maltophilia strains. Surprisingly, only rpf-1 strains produce detectable DSF, correlating with their ability to control biofilm formation, swarming motility and virulence. The evolutive advantage of acquiring two different rpf clusters, the phylogenetic time point and mechanism of this acquisition and the conditions that activate DSF production in rpf-2 strains, are however not known. Examination of this cluster in various species suggests that its variability originated most probably by genetic exchange between rhizosphere bacteria. We propose that rpf-2 variant strains make use of a strategy recently termed as "social cheating. " Analysis of cellular and extracellular fatty acids (FAs) of strains E77 (rpf-1) and M30 (rpf-2) suggests that their RpfFs have also a thioesterase activity that facilitates the release of unspecific FAs to the medium in addition to DSF. Production of DSF in rpf-1 strains appears in fact to be modulated by some of these extracellular FAs in addition to other factors such as temperature and nutrients, while in rpf-2 strains DSF biosynthesis is derepressed only upon detection of DSF itself, suggesting that they require cohabitation with DSF-producer bacteria to activate their DSF regulatory machinery. Finally, we show that the mixed rpf-1/rpf-2 population presents synergism in DSF production and virulence capacity in an in vivo infection model. Recovery and quantification of DSF from co-infected animals correlates with the observed mortality rate.
Ajuts:	European Commission 223101 Ministerio de Ciencia e Innovación BFU2010-17199 Ministerio de Economía y Competitividad AGL2012-33877 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014-SGR-1280
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	DSF bioassay ; Bacterial cross-talk ; Fatty acids ; Rpf cluster ; Social cheating ; Virulence in zebrafish
Publicat a:	Frontiers in microbiology, Vol. 6, article 761 (Jul. 2015) , p. 1-11, ISSN 1664-302X

DOI: 10.3389/fmicb.2015.00761
PMID: 26284046

11 p, 1.9 MB

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