Per citar aquest document: http://ddd.uab.cat/record/157971
Development of a transplantable glioma tumour model from genetically engineered mice : MRI/MRS/MRSI characterisation
Ciezka, Magdalena (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Acosta González, Milena (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Herranz, Cristina (Universitat de Barcelona. Departament de Ciències biomèdiques)
Canals, Josep Maria (Universitat de Barcelona. Departament de Ciències biomèdiques)
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Candiota Silveira, Ana Paula (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Arús i Caraltó, Carles (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)

Data: 2016
Resum: The initial aim of this study was to generate a transplantable glial tumour model of low-intermediate grade by disaggregation of a spontaneous tumour mass from genetically engineered models (GEM). This should result in an increased tumour incidence in comparison to GEM animals. An anaplastic oligoastrocytoma (OA) tumour of World Health Organization (WHO) grade III was obtained from a female GEM mouse with the S100β-v-erbB/inK4a-Arf (+/−) genotype maintained in the C57BL/6 background. The tumour tissue was disaggregated; tumour cells from it were grown in aggregates and stereotactically injected into C57BL/6 mice. Tumour development was followed using Magnetic Resonance Imaging (MRI), while changes in the metabolomics pattern of the masses were evaluated by Magnetic Resonance Spectroscopy/Spectroscopic Imaging (MRS/MRSI). Final tumour grade was evaluated by histopathological analysis. The total number of tumours generated from GEM cells from disaggregated tumour (CDT) was 67 with up to 100 % penetrance, as compared to 16 % in the local GEM model, with an average survival time of 66 ± 55 days, up to 4. 3-fold significantly higher than the standard GL261 glioblastoma (GBM) tumour model. Tumours produced by transplantation of cells freshly obtained from disaggregated GEM tumour were diagnosed as WHO grade III anaplastic oligodendroglioma (ODG) and OA, while tumours produced from a previously frozen sample were diagnosed as WHO grade IV GBM. We successfully grew CDT and generated tumours from a grade III GEM glial tumour. Freezing and cell culture protocols produced progression to grade IV GBM, which makes the developed transplantable model qualify as potential secondary GBM model in mice.
Nota: Número d'acord de subvenció MINECO/SAF2011–23870
Nota: Número d'acord de subvenció MINECO/SAF2012-37417
Nota: Número d'acord de subvenció MINECO/SAF2014-52332-R
Drets: Tots els drets reservats
Llengua: Anglès
Document: article ; recerca ; acceptedVersion
Matèria: Preclinical brain tumour ; Molecular imaging ; Secondary glioblastoma ; Disaggregated tumour
Publicat a: Journal of neuro-oncology, Vol. 129, issue 1 (Aug. 2016) , p. 67-76, ISSN 0167-594X

DOI: 10.1007/s11060-016-2164-3


Disponible a partir de: 2017-03-30
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