Per citar aquest document: http://ddd.uab.cat/record/163265
The small RNA content of human sperm reveals pseudogene-derived piRNAs complementary to protein-coding genes
Pantano, Lorena (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
Jodar, Meritxell (Universitat de Barcelona. Departament de Ciències Fisiològiques)
Bak, Mads (University of Copenhagen (Dinamarca))
Ballescà, Josep Lluís (Hospital Clínic i Provincial de Barcelona)
Tommerup, Niels (University of Copenhagen (Dinamarca))
Oliva, Rafael (Hospital Clínic i Provincial de Barcelona)
Vavouri, Tanya (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)

Data: 2015
Resum: Abstract. At the end of mammalian sperm development, sperm cells expel most of their cytoplasm and dispose of the majority of their RNA. Yet, hundreds of RNA molecules remain in mature sperm. The biological significance of the vast majority of these molecules is unclear. To better understand the processes that generate sperm small RNAs and what roles they may have, we sequenced and characterized the small RNA content of sperm samples from two human fertile individuals. We detected 182 microRNAs, some of which are highly abundant. The most abundant microRNA in sperm is miR-1246 with predicted targets among sperm-specific genes. The most abundant class of small noncoding RNAs in sperm are PIWI-interacting RNAs (piRNAs). Surprisingly, we found that human sperm cells contain piRNAs processed from pseudogenes. Clusters of piRNAs from human testes contain pseudogenes transcribed in the antisense strand and processed into small RNAs. Several human protein-coding genes contain antisense predicted targets of pseudogene-derived piRNAs in the male germline and these piRNAs are still found in mature sperm. Our study provides the most extensive data set and annotation of human sperm small RNAs to date and is a resource for further functional studies on the roles of sperm small RNAs. In addition, we propose that some of the pseudogene-derived human piRNAs may regulate expression of their parent gene in the male germline. © 2015 Pantano et al. ; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
Nota: MINECO/FEDER/BFU/2009-07118
Nota: MINECO/PI13/00699
Nota: Altres ajuts: MICINN grant BFU2011–30246, Ramon y Cajal grant RYC-2010-07114, European Commission Framework 7 European Re-integration grant PERG08-GA-2010-276741, Institute of Predictive and Personalized Medicine of Cancer (IMPPC), Danish Council for Independent Research (Technology and Production Sciences), The Lundbeck Foundation, Fundación Salud 2000 SERONO 13-01 i European Commission training network grant (FP7-PEOPLE-2011-ITN289880)
Drets: Tots els drets reservats
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: PIWI ; RNA ; microRNA ; piRNA ; pseudogene ; sperm
Publicat a: RNA, Vol. 21 Núm. 6 (June 2015) , ISSN 1469-9001

DOI: 10.1261/rna.046482.114


12 p, 735.8 KB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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 Registre creat el 2016-07-26, darrera modificació el 2016-10-17



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