Drosophila females undergo genome expansion after interspecific hybridization
Romero-Soriano, Valèria (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Burlet, Nelly (Université de Lyon. Laboratoire de Biométrie et Biologie evolutive)
Vela, Doris (Pontificia Universidad Católica del Ecuador. Laboratorio de Genética Evolutiva.)
Fontdevila, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Vieira, Cristina (Université de Lyon. Laboratoire de Biométrie et Biologie evolutive)
García Guerreiro, María Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2016
Resum:	Genome size (or C-value) can present a wide range of values among eukaryotes. This variation has been attributed to differences in the amplification and deletion of different noncoding repetitive sequences, particularly transposable elements (TEs). TEs can be activated under different stress conditions such as interspecific hy bridization events, as described for several species of animals and plants. These massive transposition episodes can lead to considerable genome expansions that could ultimately be involved in hybrid speciation processes. Here, we describe the effects of hybridization and introgression on genome size of Drosophila hybrids. We measured the genome size of two close Drosophila species, Drosophila buzzatiiand Drosophila koepferae,their F1 offspring and the offspring from three generations of backcrossed hybrids; where mobilization of up to 28 different TEs was previously detected. We show that hybrid females indeed present a genome expansion, especially in the first backcross, which could likely be explained bytransposition events. Hybrid males, which exhibit more variable C-values among individuals of the same generation, do not present an increased genome size. Thus, we demonstrate that the impact of hybridization on genome size can be detected through flow cytometry and is sex-dependent.
Ajuts:	Ministerio de Economía y Competitividad CGL2013-42432-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1346
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Genome size ; Flow cytometry ; Hybrids ; Drosophila ; Transposable elements ; AFLP markers
Publicat a:	Genome biology and evolution, Vol.8, Issue 3 (March 2016) , p. 556-561, ISSN 1759-6653

DOI: 10.1093/gbe/evw024
PMID: 26872773

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