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000067774 024 8_ $9 driver $9 artpubuab $a oai:ddd.uab.cat:67774
000067774 024 7_ $2 doi $a 10.1038/nchembio.243
000067774 041 __ $a eng
000067774 100 __ $a Ruiz, Amparo $u Universitat Autonòma de Barcelona. Departament de Bioquimica i Biologia Molecular
000067774 245 1_ $a Moonlighting Proteins Hal3 and Vhs3 Form a Heteromeric PPCDC with Ykl088w in Yeast CoA Biosynthesis
000067774 500 __ $a Premi a l'excel·lència investigadora. 2010
000067774 520 3_ $a Unlike most other organisms, the essential five-step Coenzyme A biosynthetic  pathway has not been fully resolved in yeast. Specifically, the gene(s) encoding the phosphopantothenoylcysteine decarboxylase (PPCDC) activity still remains unidentified. Sequence homology analyses suggest three candidates, namely Ykl088w, Hal3 and Vhs3, as putative PPCDC enzymes in Saccharomyces cerevisiae. Interestingly, Hal3 and Vhs3 have been characterized as negative regulatory subunits of the Ppz1 protein phosphatase. Here we show that YKL088w does not encode a third Ppz1 regulatory subunit, and that the essential roles of Ykl088w and the Hal3/Vhs3 pair are complementary, cannot be interchanged and can be attributed to PPCDC-related functions. We demonstrate that while known eukaryotic PPCDCs are homotrimers, the active yeast enzyme is a heterotrimer which consists of Ykl088w and Hal3/Vhs3 monomers that separately provides two essential catalytic residues. Our results unveil Hal3/Vhs3 as moonlighting proteins, involved in both CoA biosynthesis and protein phosphatase regulation.
000067774 540 __ $a Tots els drets reservats $u http://www.europeana.eu/rights/rr-f/
000067774 546 __ $a Anglès.
000067774 599 __ $a altres
000067774 653 1_ $a PREI 2010
000067774 655 _4 $a info:eu-repo/semantics/article
000067774 655 _4 $a info:eu-repo/semantics/acceptedVersion
000067774 700 __ $a González, Asier $u Universitat Autònoma de Barcelona. Institut de Biotecnologia i Biomedicina $u Universitat Autonòma de Barcelona. Departament de Bioquimica i Biologia Molecular
000067774 700 __ $a Muñoz, Ivan $u Universitat Autonòma de Barcelona. Departament de Bioquimica i Biologia Molecular
000067774 700 __ $a Serrano, Raquel $u Universitat Autonòma de Barcelona. Departament de Bioquimica i Biologia Molecular
000067774 700 __ $a Abrie, J. Albert $u Stellenbosch University (South Africa). Department of Biochemistry
000067774 700 __ $a Strauss, Erick $u Stellenbosch University (South Africa). Department of Biochemistry
000067774 700 __ $a Ariño Carmona, Joaquín $u Universitat Autonòma de Barcelona. Departament de Bioquimica i Biologia Molecular
000067774 773 __ $g Vol. 5, Núm. 12 (2009), p. 920-928 $t Nature Chemical Biology $x 1552-4469
000067774 856 40 $p 32 $s 2932955 $u http://ddd.uab.cat/pub/artpub/2009/67774/PREI2010_natchebio.pdf $z Post-print
000067774 973 __ $f 0920 $l 928 $m  $n 12 $v 5 $x v5n12 $y 2009
000067774 980 __ $a ARTPUB $b UAB $b PREI