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1.
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10 p, 2.8 MB |
A de novo evolved domain improves the cadmium detoxification capacity of limpet metallothioneins
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García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química) ;
Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Pedrini-Martha, Veronika (University of Innsbruck. Institute of Zoology) ;
Albalat, Ricard (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology)
Metallothioneins (MTs) constitute an important family of metal binding proteins. Mollusk MTs, in particular, have been used as model systems to better understand the evolution of their metal binding features and functional adaptation. [...]
2023 -  10.1038/s41598-023-35786-1
Scientific reports, Vol. 13 (June 2023) , art. 8895
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2.
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12 p, 1.5 MB |
The Modular Architecture of Metallothioneins Facilitates Domain Rearrangements and Contributes to Their Evolvability in Metal-Accumulating Mollusks
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Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química) ;
Pedrini-Martha, Veronika (University of Innsbruck. Department of Zoology) ;
Niederwanger, Michael (University of Innsbruck. Department of Zoology) ;
Dallinger, Reinhard (University of Innsbruck. Department of Zoology) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Albalat, Ricard (Universitat de Barcelona. Institut de Recerca de la Biodiversitat (IRBio))
Protein domains are independent structural and functional modules that can rearrange to create new proteins. While the evolution of multidomain proteins through the shuffling of different preexisting domains has been well documented, the evolution of domain repeat proteins and the origin of new domains are less understood. [...]
2022 - 10.3390/ijms232415824
International journal of molecular sciences, Vol. 23, Issue 24 (December 2022) , art. 15824
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3.
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14 p, 2.0 MB |
Metal-specificity divergence between Metallothioneins of Nerita peloronta (Neritimorpha, Gastropoda) sets the starting point for a novel chemical MT classification proposal
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García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química) ;
Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Pedrini-Martha, Veronika (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Albalat, Ricard (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
Metallothioneins' (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into a specific group led to numerous classification proposals. [...]
2021 - 10.3390/ijms222313114
International journal of molecular sciences, Vol. 22, Issue 23 (December 2021) , art. 13114
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4.
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15 p, 1.8 MB |
Two Unconventional Metallothioneins in the Apple Snail Pomacea bridgesii Have Lost Their Metal Specificity during Adaptation to Freshwater Habitats
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García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química) ;
Calatayud, Sara (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Niederwanger, Michael (University of Innsbruck. Institute of Zoology) ;
Albalat, Ricard (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology)
Metallothioneins (MTs) are a diverse group of proteins responsible for the control of metal homeostasis and detoxification. To investigate the impact that environmental conditions might have had on the metal-binding abilities of these proteins, we have characterized the MTs from the apple snail Pomacea bridgesii, a gastropod species belonging to the class of Caenogastropoda with an amphibious lifestyle facing diverse situations of metal bioavailability. [...]
2021 - 10.3390/ijms22010095
International journal of molecular sciences, Vol. 22, Issue 1 (January 2021) , art. 95
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5.
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13 p, 460.4 KB |
Modularity in Protein Evolution : Modular Organization and De Novo Domain Evolution in Mollusk Metallothioneins
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Calatayud, Sara (Universitat de Barcelona) ;
García-Risco, Mario (Universitat Autònoma de Barcelona. Departament de Química) ;
Pedrini-Martha, Veronika (University of Innsbruck) ;
Eernisse, Douglas J. (California State University Fullerton) ;
Dallinger, Reinhard (University of Innsbruck) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Albalat, Ricard (Universitat de Barcelona)
Metallothioneins (MTs) are proteins devoted to the control of metal homeostasis and detoxification, and therefore, MTs have been crucial for the adaptation of the living beings to variable situations of metal bioavailability. [...]
2021 - 10.1093/molbev/msaa230
Molecular biology and evolution, Vol. 38, Issue 2 (February 2021) , p. 424-436
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6.
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10 p, 2.0 MB |
Distinct pathways for zinc metabolism in the terrestrial slug Arion vulgaris
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Dvorak, Martin (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Schnegg, Raimund (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Salvenmoser, Willy (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Lindner, Herbert (Innsbruck Medical University. Institute of Clinical Biochemistry. Biocenter) ;
Zerbe, Oliver (University of Zurich. Department of Chemistry) ;
Hansel, Armin (University of Innsbruck. Institute for Ion Physics and Applied Physics) ;
Leiminger, Markus (University of Innsbruck. Institute for Ion Physics and Applied Physics) ;
Steiner, Gerhard (University of Innsbruck. Institute for Ion Physics and Applied Physics) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Lackner, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
In most organisms, the concentration of free Zn is controlled by metallothioneins (MTs). In contrast, no significant proportions of Zn are bound to MTs in the slug, Arion vulgaris. Instead, this species possesses cytoplasmic low-molecular-weight Zn (LMW Zn) binding compound that divert these metal ions into pathways uncoupled from MT metabolism. [...]
2019 - 10.1038/s41598-019-56577-7
Scientific reports, Vol. 9 (December 2019) , art. 20089
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7.
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15 p, 2.3 MB |
Biomphalaria glabrata metallothionein : Lacking metal specificity of the protein and missing gene upregulation suggest metal sequestration by exchange instead of through selective binding
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Niederwanger, Michael (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences Innsbruck) ;
Calatayud, Sara (Universitat de Barcelona. Departament de Biologia Evolutiva) ;
Zerbe, Oliver (University of Zürich. Department of Chemistry) ;
Atrian i Ventura, Sílvia (Universitat de Barcelona. Departament de Biologia Evolutiva) ;
Albalat, Ricard (Universitat de Barcelona. Departament de Biologia Evolutiva) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences Innsbruck)
The wild-type metallothionein (MT) of the freshwater snail Biomphalaria glabrata and a natural allelic mutant of it in which a lysine residue was replaced by an asparagine residue, were recombinantly expressed and analyzed for their metal-binding features with respect to Cd, Zn and Cu, applying spectroscopic and mass-spectrometric methods. [...]
2017 - 10.3390/ijms18071457
International journal of molecular sciences, Vol. 18, Num. 7 (July 2017) , art. 1457
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8.
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16 p, 2.3 MB |
Analysis of metal-binding features of the wild type and two domain-truncated mutant variants of Littorina littorea metallothionein reveals its cd-specific character
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Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Jiménez Martí, Elena (Universitat de Barcelona. Departament de Genètica) ;
Niederwanger, Michael (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Gil-Moreno, Selene (Universitat Autònoma de Barcelona. Departament de Química) ;
Zerbe, Oliver (University of Zurich. Institute of Organic Chemistry) ;
Atrian i Ventura, Sílvia (Universitat de Barcelona. Departament de Genètica) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
After the resolution of the 3D structure of the Cd9-aggregate of the Littorina littorea metallothionein (MT), we report here a detailed analysis of the metal binding capabilities of the wild type MT, LlwtMT, and of two truncated mutants lacking either the N-terminal domain, Lltr2MT, or both the N-terminal domain, plus four extra flanking residues (SSVF), Lltr1MT. [...]
2017 - 10.3390/ijms18071452
International journal of molecular sciences, Vol. 18 Núm. 7 (july 2017) , p. 1452
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9.
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13 p, 1.3 MB |
Does Variation of the Inter-Domain Linker Sequence Modulate the Metal Binding Behaviour of Helix pomatia Cd-Metallothionein?
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Gil-Moreno, Selene (Universitat Autònoma de Barcelona. Departament de Química) ;
Jiménez Martí, Elena (Universitat de Barcelona. Departament de Genètica) ;
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Zerbe, Oliver (University of Zurich. Institute of Organic Chemistry) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Atrian i Ventura, Sílvia (Universitat de Barcelona. Departament de Genètica)
Snail metallothioneins (MTs) constitute an ideal model to study structure/function relationships in these metal-binding polypeptides. Helix pomatia harbours three MT isoforms: the highly specific CdMT and CuMT, and an unspecific Cd/CuMT, which represent paralogous proteins with extremely different metal binding preferences while sharing high sequence similarity. [...]
2016 - 10.3390/ijms17010006
International journal of molecular sciences, Vol. 17 Núm. 6 (2016) , p. 1-13
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10.
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20 p, 2.3 MB |
Shaping mechanisms of metal specificity in a family of metazoan metallothioneins : evolutionary differentiation of mollusc metallothioneins
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Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química) ;
Pagani, Ayelen (Universitat de Barcelona. Departament de Genètica) ;
Pérez Rafael, Sílvia (Universitat Autònoma de Barcelona. Departament de Química) ;
Egg, Margit (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Höckner, Martina (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences) ;
Brandstätter, Anita (Innsbruck Medical University. Department of Medical Genetics) ;
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química) ;
Atrian i Ventura, Sílvia (Universitat de Barcelona. Departament de Genètica) ;
Dallinger, Reinhard (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences)
Background: The degree of metal binding specificity in metalloproteins such as metallothioneins (MTs) can be crucial for their functional accuracy. Unlike most other animal species, pulmonate molluscs possess homometallic MT isoforms loaded with Cu+ or Cd2+. [...]
2011 - 10.1186/1741-7007-9-4
BMC biology, Vol. 9, Núm. 4 (January 2011) , p. 1-20
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