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13 p, 2.6 MB |
Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein production bioprocesses
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Sales-Vallverdú, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gasset Franch, Arnau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Requena-Moreno, Guillermo (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The yeast Komagataella phaffii (Pichia pastoris) is currently considered a versatile and highly efficient host for recombinant protein production (RPP). Interestingly, the regulated application of specific stress factors as part of bioprocess engineering strategies has proven potential for increasing the production of recombinant products. [...]
2024 -  10.1111/1751-7915.14411
Microbial biotechnology, Vol. 17, Issue 2 (February 2024) , art. e14411
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2.
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28 p, 460.0 KB |
Enzymatic production of biodiesel : Strategies to overcome methanol inactivation
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Lotti, Marina (Università degli Studi Milano-Bicocca. Dipartimento di Biotecnologie e Bioscienze) ;
Pleiss, Jürgen (University of Stuttgart. Institute of Biochemistry and Technical Biochemistry) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lipase-catalyzed transesterification of triglycerides and alcohols to obtain biodiesel is an environmentally friendly and sustainable route for fuels production since, besides proceeding in mild reaction conditions, it allows for the use of low-cost feedstocks that contain water and free fatty acids, for example non-edible oils and waste oils. [...]
2018 - 10.1002/biot.201700155
Biotechnology Journal, Vol. 13, Issue 5 (May 2018) , art. 1700155
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3.
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12 p, 1.2 MB |
Enzymatic Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor
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Holtheuer, Jessica (Pontificia Universidad Católica de Valparaíso) ;
Tavernini, Luigi (Pontificia Universidad Católica de Valparaíso) ;
Bernal, Claudia (Universidad de La Serena) ;
Romero, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ottone, Carminna (Pontificia Universidad Católica de Valparaíso) ;
Wilson, Lorena (Pontificia Universidad Católica de Valparaíso)
Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. [...]
2023 - 10.3390/molecules28020644
Molecules, Vol. 28, Num. 2 (January 2023) , art. 644
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4.
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13 p, 1.7 MB |
Producing natural flavours from isoamyl alcohol and fusel oil by using immobilised Rhizopus oryzae lipase
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Sebastian, Xavier (Hausmann, S.L.) ;
Bueno, Jose María (Hausmann, S.L.) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Enzymatic synthesis of short-chain esters (flavours) might enable their labelling as natural, increasing their value. Covalently immobilised Rhizopus oryzae lipase (EO-proROL) was used to synthesise isoamyl butyrate and acetate. [...]
2022 - 10.3390/catal12060639
Catalysts, Vol. 12, Issue 6 (June 2022) , art. 639
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5.
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10 p, 4.6 MB |
Near Infrared Spectroscopy : A useful technique for inline monitoring of the enzyme catalyzed biosynthesis of third-generation biodiesel from waste cooking oil
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Moya, Desireè (Universitat Autònoma de Barcelona. Departament de Química) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Alcalà Cañadas, Ma. del Mar (Universitat Autònoma de Barcelona. Departament de Química)
Near Infrared (NIR) spectroscopy was used for inline monitoring the enzymatic production of third-generation biodiesel from waste cooking oil (WCO) -with fatty acid profile similar to olive oil- under the principles of Continued Process Verification (CPV) and Process Analytical Technology (PAT). [...]
2022 - 10.1016/j.fuel.2022.123794
Fuel, Vol. 319 (July 2022) , art. 123794
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6.
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15 p, 900.6 KB |
Constitutive expression in komagataella phaffii of mature rhizopus oryzae lipase jointly with its truncated prosequence improves production and the biocatalyst operational stability
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rhizopus oryzae lipase (ROL) containing 28 C-terminal amino acids of the prosequence fused to the N-terminal mature sequence in ROL (proROL) was successfully expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris) under the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP). [...]
2021 - 10.3390/catal11101192
Catalysts, Vol. 11, Issue 10 (October 2021) , art. 1192
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7.
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9 p, 4.2 MB |
Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase : Influence of the support, substrate acidity and bioprocess scale-up
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace oil. [...]
2021 - 10.1016/j.biortech.2021.125233
Bioresource Technology, Vol. 334 (August 2021) , art. 125233
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8.
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50 p, 2.4 MB |
Rhizopus oryzae lipase, a promising industrial enzyme : biochemical characteristics, production and biocatalytic applications
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lipases are biocatalysts with a significant potential to enable a shift from current pollutant manufacturing processes to environmentally sustainable approaches. The main reason of this prospect is their catalytic versatility as they carry out several industrially relevant reactions as hydrolysis of fats in water/lipid interface and synthesis reactions in solvent-free or non-aqueous media such as transesterification, interesterification and esterification. [...]
2020 - 10.3390/catal10111277
Catalysts, Vol. 10, Issue 11 (November 2020) , art. 1277
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9.
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16 p, 2.6 MB |
Truncated prosequence of rhizopus oryzae lipase : key factor for production improvement and biocatalyst stability
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Barrero Peña, Juan José (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Recombinant Rhizopus oryzae lipase (mature sequence, rROL) was modified by adding to its N-terminal 28 additional amino acids from the C-terminal of the prosequence (proROL) to obtain a biocatalyst more suitable for the biodiesel industry. [...]
2019 - 10.3390/catal9110961
Catalysts, Vol. 9, issue 11 (Nov. 2019) , art. 961
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10.
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28 p, 709.9 KB |
Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis
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Bonet Ragel, Kírian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentration. [...]
2018 - 10.1002/jctb.5399
Journal of chemical technology and biotechnology, Vol. 93, issue 2 (Feb. 2018) , p. 541-547
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