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Title:          Notch-mediated induction of N-cadherin and α9-integrin confers higher invasive phenotype on rhabdomyosarcoma cells
Subject:        British Journal of Cancer 107, 1374 (2012). doi:10.1038/bjc.2012.411
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        Title: Materials and Methods
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          Title: Cell culture, transfections and drug treatments
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          Title: Complementary DNA microarrays
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          Title: Pharmacological inhibition of Notch signalling impairs cell adhesion in RMS cell lines
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          Title: Pharmacological inhibition of Notch signalling significantly reduces N- cadherin and agr9-integrin expression
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          Title: Genetic modification of Notch pathway activation gives rise to a parallel regulation of N- cadherin and agr9-integrin
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        Title: Figure™1Cell adhesion to fibronectin- or collagen-coated plates. Images showing a representative field of attached cells for each cell line in the presence (GSI) or absence (DMSO) of gamma-secretase inhibitor and plots representing the quantification of c
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          Title: Immunofluorescence
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          Title: Specific anti-N-cadherin- and anti-agr9-integrin-blocking antibodies reduce invasive properties of RMS cells
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        Title: Figure™2Pharmacological inhibition of Notch signalling significantly reduced N- cadherin and agr9-integrin expression. (A) Complementary DNA (cDNA) expression microarray showing the relative levels of agr9-integrin (ITGA9), N- cadherin (N-Cad) and Hes1 ex
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          Title: Correlation among Hes1, N- cadherin and agr9-integrin in RMS archival tumour samples
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          Title: NICD and Hes1 bind to N- cadherin and agr9-integrin promoters
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        Title: Discussion
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        Title: Figure™3Genetic modifications of the Notch pathway influenced N- cadherin and agr9-integrin expression. Western blots showing the concomitant variations of Notch pathway activation (NICD and Hes1) and N- cadherin (N-CadsolFL and CTF2) and agr9-integrin (I
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        Title: Figure™4N-cadherin and agr9-integrin immunocytochemistry. Immunocytochemistry with anti-Hes1 and N- cadherin (N-Cad) antibodies (A) or Hes1 and agr9-integrin (ITGA9) antibodies (B) in cells transfected with Delta or control vector (CTRL). Staining was as 
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        Title: Figure™5Anti-N-cadherin- and anti-agr9-integrin-blocking antibodies reduced invasive properties induced by Notch activation. Three RMS cell lines were transfected with the constructions indicated below the bottom of each bar (pCDNA, Delta) in the presence
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        Title: ACKNOWLEDGEMENTS
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        Title: Figure™6The expression of Hes1, N- cadherin and agr9-integrin correlate in archival RMS tumour samples. (A) Representative sections of three patients (34, 37 and 41) stained with anti-Hes1, N- Cad and agr9-integrin antibodies. (B and C) Plots depicting th
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        Title: Figure™7Binding of NICD and Hes1 to N- cadherin and agr9-integrin promoters. Lysates from RH30 cells were analysed by ChIP assay using specific antibodies against Hes1, NICD and rabbit IgG as a control. Immunoprecipitated chromatin was amplified by PCR us
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