6564dee9f73672735adfd8033379b25b Celrep_a2013m7v4n2p362.pdf c10bafbc71703fe4fd58e67bd844950b36601201 Celrep_a2013m7v4n2p362.pdf d964eaf0c9501db66bf4f6e789915f81ab7d27ba5e2d2fa2ab8c2b967db1f547 Celrep_a2013m7v4n2p362.pdf Title: SUMO-2 and PIAS1 Modulate Insoluble Mutant Huntingtin Protein Accumulation Subject: CellReports, 4 (2013) 362-375. doi:10.1016/j.celrep.2013.06.034 Author: Jacqueline Gire O’Rourke Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Thu Jul 18 22:52:38 2013 CEST ModDate: Fri Jul 19 05:56:37 2013 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 602.986 x 782.986 pts Page rot: 0 File size: 2372385 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PHCNCM+AdvPSHN-M Type 1C Custom yes yes no 700 0 PHCNDN+AdvPSHN-H Type 1C MacRoman yes yes no 701 0 PHCNDO+AdvPSA189 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DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: SUMO-2 and PIAS1 Modulate Insoluble Mutant Huntingtin Protein Accumulation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7c53a9eb Children: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed17bee Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2a33fae0 Children: Item: Title: HTTex1p Lys 6 and Lys 9 Are the Primary Sites of SUMO Modification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@707f7052 Item: Title: PIAS and SUMO Modification Proteins Are Highly Expressed in Mouse Brain Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11028347 Item: Title: HTTex1p Is Modified by Both SUMO-1 and SUMO-2 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@14899482 Item: Title: PIAS1 Is a Candidate SUMO E3 Ligase for HTT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@21588809 Item: Title: Longer HTT Fragments Are SUMOylated Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2aae9190 Item: Title: PIAS1 Increases SUMO-2 Modification of HTT 586 aa Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2f333739 Item: Title: SUMO-2 Promotes Accumulation of Insoluble Mutant HTT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77468bd9 Item: Title: Genetic Reduction of Su(-var)2-10 Is Neuroprotective in Mutant HTTex1p-Expressing Drosophila Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12bb4df8 Item: Title: Insoluble SUMO-2-Modified Proteins Are Increased in Human HD Brain Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4cc77c2e Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a7b0070 Item: Title: Experimental Procedures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@39a054a5 Children: Item: Title: Plasmids Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@71bc1ae4 Item: Title: siRNA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6ed3ef1 Item: Title: Primary Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2437c6dc Item: Title: GST Pull-Down Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1f89ab83 Item: Title: In Vitro SUMO Modification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e73f9ac Item: Title: Mass Spectrometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@61064425 Item: Title: Cell Culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b1d7fff Item: Title: Western Blot Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@299a06ac Item: Title: Nickel Purifications Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@383534aa Item: Title: Immunoprecipitations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6bc168e5 Item: Title: Soluble/Insoluble Fractionation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b3300e5 Item: Title: Filter Retardation Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2e5c649 Item: Title: RNA Isolation and Real-Time Quantitative PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@136432db Item: Title: Automated Y2H Screening Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7382f612 Item: Title: Drosophila Crosses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1055e4af Item: Title: Human Postmortem Brain Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3caeaf62 Item: Title: Supplemental Information Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e6ea0c6 Item: Title: Acknowledgments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a38e57f Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5577140b Info: Title: SUMO-2 and PIAS1 Modulate Insoluble Mutant Huntingtin Protein Accumulation Author: Jacqueline Gire O’Rourke Subject: CellReports, 4 (2013) 362-375. doi:10.1016/j.celrep.2013.06.034 Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Thu Jul 18 23:22:38 CEST 2013 ModDate: Fri Jul 19 06:26:37 CEST 2013 ID: 0xeb04e13bdc5483640ff4764e95e1752f, 0x7a379b06bd404b4fb8b18f5e0399d072 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: CompressionScheme: JPEG ImageWidth: 83 ImageHeight: 83 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: ImageWidth: 893 ImageHeight: 349 ColorSpace: palette color BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: 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PHCNCM+AdvPSHN-M Flags: Nonsymbolic FontBBox: -156, -218, 1062, 802 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: PHCNDN+AdvPSHN-H FontSubset: true FirstChar: 40 LastChar: 222 FontDescriptor: FontName: PHCNDN+AdvPSHN-H Flags: Nonsymbolic FontBBox: -166, -229, 1093, 812 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: PHCNDO+AdvPSA189 FontSubset: true FirstChar: 45 LastChar: 222 FontDescriptor: FontName: PHCNDO+AdvPSA189 Flags: Nonsymbolic FontBBox: -156, -208, 1072, 802 FontFile3: true Encoding: MacRomanEncoding XMP: application/pdf doi:10.1016/j.celrep.2013.06.034 SUMO-2 and PIAS1 Modulate Insoluble Mutant Huntingtin Protein Accumulation Jacqueline Gire O’Rourke Jaclyn R. Gareau Joseph Ochaba Wan Song Tamás Raskó David Reverter John Lee Alex Mas Monteys Judit Pallos Lisa Mee Malini Vashishtha Barbara L. Apostol Thomas Peter Nicholson Katalin Illes Ya-Zhen Zhu Mary Dasso Gillian P. Bates Marian Difiglia Beverly Davidson Erich E. Wanker J. Lawrence Marsh Christopher D. Lima Joan S. Steffan Leslie M. Thompson CellReports, 4 (2013) 362-375. doi:10.1016/j.celrep.2013.06.034 The Authors journal CellReports Copyright  © 2013 The Authors. Published by Elsevier Ltd. All rights reserved 2211-1247 4 2 25 July 2013 2013-07-25 362-375 362 375 10.1016/j.celrep.2013.06.034 http://dx.doi.org/10.1016/j.celrep.2013.06.034 2010-04-23 true 10.1016/j.celrep.2013.06.034 elsevier.com sciencedirect.com e26639d8-fbb4-4ffb-9e9c-5db8cd36db01:1559219341 10.1016/j.celrep.2013.06.034 noindex 2010-04-23 true sciencedirect.com elsevier.com Elsevier 2013-07-19T02:22:38+05:30 2013-07-19T09:26:37+05:30 2013-07-19T09:26:37+05:30 True Acrobat Distiller 8.1.0 (Windows) uuid:ec2c6b6a-8e6c-4fab-9993-364506ed4ad1 uuid:d3f82179-e979-4549-9ce2-4b570b982ba2 Pages: Page: Label: 362 Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 168, 618, 172, 627 Destination: 617 Annotation: Subtype: Link Rect: 173, 618, 177, 627 Destination: 617 Annotation: Subtype: Link Rect: 179, 618, 183, 627 Destination: 617 Annotation: Subtype: Link Rect: 258, 618, 262, 627 Destination: 617 Annotation: Subtype: Link Rect: 332, 618, 336, 627 Destination: 617 Annotation: Subtype: Link Rect: 338, 618, 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