41f81e8cd37b2ec83628e0988378363d miccelfac_a2014v13n36ahemmerich.pdf 0df312f69764ee5f16ecb1bf3488e0cb58aa43b8 miccelfac_a2014v13n36ahemmerich.pdf bf2c201b09d4434b598fc3c4454df3b53fb1d2388db4e5474496f919951a5c44 miccelfac_a2014v13n36ahemmerich.pdf Title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.1.5 (Windows) CreationDate: Thu Mar 27 13:52:42 2014 CET ModDate: Thu Mar 27 13:53:15 2014 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 1095672 bytes Optimized: no PDF version: 1.3 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ODILHD+AdvTTaf7f9f4f.B Type 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@449b2d27 Item: Title: Background Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5479e3f Item: Title: Results and discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@27082746 Children: Item: Title: Clone selection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66133adc Item: Title: Microbioreactor cultivation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7bfcd12c Children: Item: Title: Effects of cultivation media in clone screening Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42f30e0a Item: Title: Microbioreactor for evaluation of operational fed-batch strategies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@24273305 Item: Title: Strategy 1: enzymatic continuous glucose feeding with MeOH induction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5b1d2887 Item: Title: Strategy 2: pulsed feeding of glycerol/MeOH Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46f5f779 Children: Item: Title: Comparison of clone rankings for different fed-batch strategies in microbioreactor system Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1c2c22f3 Item: Title: Lab scale bioreactor cultivations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@18e8568 Children: Item: Title: Comparison of operational fed-batch strategies and scales Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33e5ccce Item: Title: Conclusions & outlook Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5a42bbf4 Item: Title: Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@270421f5 Children: Item: Title: Organisms Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52d455b8 Item: Title: Cultivation media Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4f4a7090 Item: Title: Shake flasks cultivations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@18ef96 Item: Title: Microbioreactor cultivations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6956de9 Item: Title: Bioreactor cultivations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@769c9116 Item: Title: Biomass analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6aceb1a5 Item: Title: Lipolytic activity assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2d6d8735 Item: Title: Determination of oxygen transfer rates in microbioreactor system Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ba4d54 Item: Title: Additional file Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12bc6874 Item: Title: Abbreviations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@de0a01f Item: Title: Competing interests Destination: 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Pichia pastoris Clone screening Rhizopus oryzae lipase Fed-batch fermentation Feeding strategies Microbioreactor Scale-up Bioprocess development True Microbial Cell Factories 10.1186/1475-2859-13-36 1-16 1 16 � 2014 Hemmerich et al.; licensee BioMed Central Ltd. Microbial Cell Factories journal 13 reprints@biomedcentral.com 2014-03-07 1 1 Microbial Cell Factories Pichia pastoris; Clone screening; Rhizopus oryzae lipase; Fed-batch fermentation; Feeding strategies; Microbioreactor; Scale-up; Bioprocess development Pages: Page: Label: 0 Annotations: Annotation: Subtype: Link Rect: 114, 137, 178, 144 Annotation: Subtype: Link Rect: 182, 137, 256, 144 Annotation: Subtype: Link Rect: 287, 67, 403, 74 Annotation: Subtype: Link Rect: 255, 51, 394, 58 Thumb: true Page: Label: 1 Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 249, 526, 254, 536 Destination: 199 Annotation: Subtype: Link Rect: 258, 526, 263, 536 Destination: 199 Annotation: Subtype: Link Rect: 149, 490, 154, 500 Destination: 199 Annotation: Subtype: Link Rect: 176, 406, 181, 416 Destination: 199 Annotation: Subtype: Link Rect: 202, 370, 207, 380 Destination: 199 Annotation: Subtype: Link Rect: 105, 334, 110, 344 Destination: 199 Annotation: Subtype: Link Rect: 113, 334, 118, 344 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