1aa01223feff2744b263fc4e0ebea89c joumarscieeng_a2015v3p1334.pdf df38ae08e8b671b083d8380814efd35c0b2d7523 joumarscieeng_a2015v3p1334.pdf ee1ff34c5579c8a97cdad3c974932ff0415c6e0ed9bddfd80d8d9887078e4cf4 joumarscieeng_a2015v3p1334.pdf Title: Human Genotoxic Study Carried Out Two Years after Oil Exposure during the Clean-up Activities Using Two Different Biomarkers Subject: Micronuclei, comet and chromosome alterations assays are the most widely used biomarkers for determining the genotoxic damage in a population exposed to genotoxic chemicals. While chromosome alterations are an excellent biomarker to detect short- and long-term genotoxic effects, the comet assay only measures early biological effects, and furthermore it is unknown whether nuclear abnormalies, such as those measured in the micronucleus test, remain detectable long-term after an acute exposure. In our previous study, an increase in structural chromosome alterations in fishermen involved in the clean-up of the Prestige oil spill, two years after acute exposure, was detected. The aim of this study is to investigate whether, in lymphocytes from peripheral blood, the nuclear abnormalies (micronucleus, nucleoplasmic bridges and nuclear buds) have a similar sensitivity to the chromosome damage analysis for genotoxic detection two years after oil exposure in the same non-smoker individuals and in the same peripheral blood extraction. No significant differences in nuclear abnormalies frequencies between exposed and non-exposed individuals were found (p > 0.05). However, chromosome damage, in the same individuals, was higher in exposed vs. non-exposed individuals, especially for chromosome lesions (p < 0.05). These findings, despite the small sample size, suggest that nuclear abnormalities are probably less-successful biomarkers than are chromosome alterations to evaluate genotoxic effects two or more years after an exposure to oil. Due to the great advantage of micronucleus automatic determination, which allows for a rapid study of hundreds of individuals exposed to genotoxic chemical exposure, further studies are needed to confirm whether this assay is or is not useful in long-term genotoxic studies after the toxic agent is no longer present. Keywords: micronucleus test; chromosome damage; nuclear abnormities; chromosome alterations; oil exposure; genotoxicity; Prestige catastrophe Author: Gloria Biern, Jesús Giraldo, Jan-Paul Zock, Gemma Monyarch, Ana Espinosa, Gema Rodríguez-Trigo, Federico Gómez, Francisco Pozo-Rodríguez, Joan-Albert Barberà and Carme Fuster Creator: PScript5.dll Version 5.2.2 Producer: Acrobat Distiller 11.0 (Windows) CreationDate: Tue Nov 3 15:38:43 2015 ModDate: Tue Feb 9 13:29:03 2016 Tagged: no Pages: 15 Encrypted: no Page size: 595.22 x 842 pts (A4) File size: 1288684 bytes Optimized: yes PDF version: 1.6 name type emb sub uni object ID ------------------------------------ ----------------- --- --- --- --------- HONGJB+ArialMT TrueType yes yes no 104 0 HONGEL+TimesNewRomanPS-ItalicMT TrueType yes yes no 106 0 HONGFN+TimesNewRomanPSMT TrueType yes yes no 108 0 HONGGO+TimesNewRomanPS-BoldMT TrueType yes yes no 110 0 HONGIA+TimesNewRomanPS-BoldItalicMT TrueType yes yes no 112 0 HONGGN+TimesNewRomanPS-BoldMT CID TrueType yes yes yes 76 0 HONNIF+SymbolMT CID TrueType yes yes yes 77 0 Jhove (Rel. 1.6, 2011-01-04) Date: 2016-02-12 07:38:03 GMT+01:00 RepresentationInformation: joumarscieeng_a2015v3p1334.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2016-02-09 13:29:32 GMT+01:00 Size: 1288684 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: da69a210 Type: CRC32 Checksum: 1aa01223feff2744b263fc4e0ebea89c Type: MD5 Checksum: df38ae08e8b671b083d8380814efd35c0b2d7523 Type: SHA-1