019ab7dc784773d958afb280aab2c0dd sensors_a2015v15p2798.pdf 798d9eef6b373f5fdd1375263dce7ad7a73b1a1a sensors_a2015v15p2798.pdf 6e3e7fb88a70f46c872a7eecb34a0502b9b35e910b386f1fa61128c2ab545618 sensors_a2015v15p2798.pdf Title: Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate Subject: Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05–1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed. Keywords: "monitoring; lipase/esterase activity; sequential injection analysis; stopped flow; p-nitrophenyl esters" Author: Jorge Pliego, Juan Carlos Mateos, Jorge Rodriguez, Francisco Valero, Mireia Baeza, Ricardo Femat, Rosa Camacho, Georgina Sandoval, Enrique J. Herrera-López Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.13 CreationDate: Tue Jan 27 16:50:09 2015 ModDate: Tue Feb 16 11:24:26 2016 Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3853220 bytes Optimized: yes PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZMXQOM+CMBX8 Type 1 Builtin yes yes no 347 0 RTKTVU+CMMIB8 Type 1 Builtin yes yes no 350 0 QTKFEK+CMR8 Type 1 Builtin yes yes no 353 0 XYISLN+NimbusRomNo9L-ReguItal Type 1 Custom yes yes no 357 0 JRSUBF+NimbusRomNo9L-Medi Type 1 Custom yes yes no 360 0 LTDTPF+NimbusRomNo9L-Regu Type 1 Custom yes yes no 363 0 IHXDYJ+NimbusRomNo9L-MediItal Type 1 Custom yes yes no 366 0 HWKEHN+EURM10 Type 1 Builtin yes yes no 170 0 BJHJVY+CMSY8 Type 1 Builtin yes yes no 174 0 FVOJAZ+CMR12 Type 1 Builtin yes yes no 176 0 GFOSLW+CMMI12 Type 1 Builtin yes yes no 180 0 Times-Roman Type 1 Standard no no no 113 0 Times-Bold Type 1 Standard no no no 114 0 Helvetica Type 1 Standard no no no 115 0 Times-Italic Type 1 Standard no no no 116 0 TWSTOL+CMSY10 Type 1 Builtin yes yes no 182 0 PUGVIF+CMMI10 Type 1 Builtin yes yes no 185 0 TXSQYF+CMTI12 Type 1 Builtin yes yes no 187 0 MWSKNY+CMBSY8 Type 1 Builtin yes yes no 189 0 CZOINJ+EURB10 Type 1 Builtin yes yes no 190 0 Jhove (Rel. 1.6, 2011-01-04) Date: 2016-02-17 07:25:33 CET RepresentationInformation: sensors_a2015v15p2798.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2016-02-16 11:27:55 CET Size: 3853220 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: 93685a8a Type: CRC32 Checksum: 019ab7dc784773d958afb280aab2c0dd Type: MD5 Checksum: 798d9eef6b373f5fdd1375263dce7ad7a73b1a1a Type: SHA-1