27f5275b86c9c91d4df82c277d34d0ea biology_a2015m2v4p67.pdf 9fefee8b8deacf9b1b7a45dd96b9b13b5ef875f6 biology_a2015m2v4p67.pdf 05de8e57a2dc830bd1772c2798a7222a7e641b701121e478baa7862e91062842 biology_a2015m2v4p67.pdf Title: In Vivo Molecular Responses of Fast and Slow Muscle Fibers to Lipopolysaccharide in a Teleost Fish, the Rainbow Trout (Oncorhynchus mykiss) Subject: The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout. Keywords: muscle; lipopolysaccharide; transcriptome; proteome; fish Author: Leonardo J. Magnoni 1,†, Nerea Roher 2,†, Diego Crespo 1, Aleksei Krasnov 3 and Creator: PScript5.dll Version 5.2.2 Producer: Acrobat Distiller 9.0.0 (Windows) CreationDate: Tue Feb 3 10:06:53 2015 ModDate: Tue Feb 3 10:08:14 2015 Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.22 x 842 pts (A4) Page rot: 0 File size: 692242 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FGNKMH+TimesNewRomanPS-ItalicMT Type 1C WinAnsi yes yes no 111 0 FGNKMI+TimesNewRomanPS-BoldMT Type 1C WinAnsi yes yes no 112 0 FGNKMJ+TimesNewRomanPSMT Type 1C Custom yes yes yes 113 0 FGNKMK+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 114 0 Jhove (Rel. 1.6, 2011-01-04) Date: 2016-11-22 02:34:16 CET RepresentationInformation: biology_a2015m2v4p67.pdf ReportingModule: PDF-hul, Rel. 1.8 (2009-05-22) LastModified: 2016-11-21 15:27:40 CET Size: 692242 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: ISO PDF/A-1, Level B PDFMetadata: Objects: 132 FreeObjects: 2 IncrementalUpdates: 2 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Info: Title: In Vivo Molecular Responses of Fast and Slow Muscle Fibers to Lipopolysaccharide in a Teleost Fish, the Rainbow Trout (Oncorhynchus mykiss) Author: Leonardo J. Magnoni 1,†, Nerea Roher 2,†, Diego Crespo 1, Aleksei Krasnov 3 and Subject: The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout. Keywords: muscle; lipopolysaccharide; transcriptome; proteome; fish Creator: PScript5.dll Version 5.2.2 Producer: Acrobat Distiller 9.0.0 (Windows) CreationDate: Tue Feb 03 03:06:53 CET 2015 ModDate: Tue Feb 03 03:08:14 CET 2015 ID: 0xbbda5dce3893fe75191e9487e81477cf, 0x01bc94c15916df43b24b688039e9db86 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: ImageWidth: 833 ImageHeight: 232 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 833 ImageHeight: 232 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 833 ImageHeight: 232 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 932 ImageHeight: 218 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 932 ImageHeight: 218 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 932 ImageHeight: 219 ColorSpace: CMYK BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 932 ImageHeight: 218 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 919 ImageHeight: 216 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: CompressionScheme: JPEG ImageWidth: 919 ImageHeight: 216 ColorSpace: CMYK BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: ImageWidth: 919 ImageHeight: 216 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 919 ImageHeight: 216 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 1015 ImageHeight: 727 BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode ImageMask: true Image: NisoImageMetadata: ImageWidth: 1639 ImageHeight: 1127 BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode ImageMask: true Image: NisoImageMetadata: ImageWidth: 1079 ImageHeight: 743 BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode ImageMask: true Image: NisoImageMetadata: ImageWidth: 1175 ImageHeight: 903 BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode ImageMask: true Image: NisoImageMetadata: CompressionScheme: JPEG ImageWidth: 968 ImageHeight: 253 ColorSpace: CMYK BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: ImageWidth: 968 ImageHeight: 253 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: ImageWidth: 968 ImageHeight: 253 ColorSpace: CMYK BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type1: Font: BaseFont: FGNKMK+TimesNewRomanPS-BoldItalicMT FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: FGNKMK+TimesNewRomanPS-BoldItalicMT Flags: Serif, Nonsymbolic, Italic FontBBox: -547, -307, 1401, 1032 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: FGNKMJ+TimesNewRomanPSMT FontSubset: true FirstChar: 2 LastChar: 250 FontDescriptor: FontName: FGNKMJ+TimesNewRomanPSMT Flags: Serif, Symbolic FontBBox: -568, -307, 2000, 1007 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: FGNKMI+TimesNewRomanPS-BoldMT FontSubset: true FirstChar: 32 LastChar: 134 FontDescriptor: FontName: FGNKMI+TimesNewRomanPS-BoldMT Flags: Nonsymbolic FontBBox: -558, -307, 2000, 1026 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: FGNKMH+TimesNewRomanPS-ItalicMT FontSubset: true FirstChar: 32 LastChar: 122 FontDescriptor: FontName: FGNKMH+TimesNewRomanPS-ItalicMT Flags: Serif, Nonsymbolic, Italic FontBBox: -498, -307, 1333, 1023 FontFile3: true Encoding: WinAnsiEncoding XMP: 2015-02-03T10:06:53+08:00 PScript5.dll Version 5.2.2 2015-02-03T10:08:14+08:00 2015-02-03T10:08:14+08:00 Acrobat Distiller 9.0.0 (Windows) muscle; lipopolysaccharide; transcriptome; proteome; fish application/pdf In Vivo Molecular Responses of Fast and Slow Muscle Fibers to Lipopolysaccharide in a Teleost Fish, the Rainbow Trout (Oncorhynchus mykiss) Leonardo J. Magnoni 1,†, Nerea Roher 2,†, Diego Crespo 1, Aleksei Krasnov 3 and The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout. muscle lipopolysaccharide transcriptome proteome fish uuid:bbb56217-8e10-41bc-a1a1-8c3a1833818f uuid:7d7b3faa-d310-46c9-9cc1-83762d5cd2e9 Pages: Page: Label: 1 Page: Label: 2 Page: Label: 3 Page: Label: 4 Page: Label: 5 Page: Label: 6 Page: Label: 7 Page: Label: 8 Page: Label: 9 Page: Label: 10 Page: Label: 11 Page: Label: 12 Page: Label: 13 Page: Label: 14 Page: Label: 15 Page: Label: 16 Page: Label: 17 Page: Label: 18 Page: Label: 19 Page: Label: 20 Page: Label: 21 Checksum: a091b493 Type: CRC32 Checksum: 27f5275b86c9c91d4df82c277d34d0ea Type: MD5 Checksum: 9fefee8b8deacf9b1b7a45dd96b9b13b5ef875f6 Type: SHA-1