23e9933d54f711821e62c85f1b8719fb 300281.pdf b315b6476ccb4bad39675b2e8779ab40fc082b1a 300281.pdf 1f4546ea2013386040b4f81191a99fd8d4454d3e0d2f41c6b590d2b64b194ae8 300281.pdf Title: A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR Subject: Leukemia 29, 369 (2015). doi:10.1038/leu.2014.217 Author: H White Creator: Arbortext Advanced Print Publisher 10.0.1465/W Unicode Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Fri Jan 2 18:00:52 2015 CET ModDate: Fri Jan 16 15:42:39 2015 CET Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 824961 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BLMMDB+AdvOT65f8a23b.I+20 Type 1C WinAnsi yes yes no 289 0 BLMMEC+AdvOT46dcae81+03 Type 1C Custom yes yes yes 303 0 BLMMED+AdvOT3b30f6db.B Type 1C WinAnsi yes yes no 304 0 BLMMEE+AdvOT46dcae81+20 Type 1C WinAnsi yes yes no 305 0 BLMLLJ+Arial-BoldMT Type 1C WinAnsi yes yes no 276 0 BLMMBI+AdvTT5afe5d37.B Type 1C WinAnsi yes yes no 277 0 BLMMBJ+AdvOTa20b42a7 Type 1C WinAnsi yes yes no 278 0 BLMMBK+AdvOTa20b42a7+fb Type 1C Custom yes yes yes 279 0 BLMMBL+AdvOT5cf73554.I Type 1C WinAnsi yes yes no 280 0 BLMMBM+AdvOT5cf73554.I+20 Type 1C WinAnsi yes yes no 281 0 BLMMBN+AdvTT6780a46b Type 1C WinAnsi yes yes no 282 0 BLMMBO+AdvOT8608a8d1+01 Type 1C Custom yes yes yes 283 0 BLMMCO+AdvOT46dcae81 Type 1C WinAnsi yes yes no 292 0 BLMMCP+AdvOT46dcae81+fb Type 1C Custom yes yes yes 293 0 BLMMDA+AdvOT65f8a23b.I Type 1C WinAnsi yes yes no 294 0 BLMPGC+AdvOT8608a8d1+2a Type 1C Custom yes yes yes 133 0 BLNMFD+AdvOT919a0533.B Type 1C WinAnsi yes yes no 134 0 BLNNPK+AdvOT7d2ebc01 Type 1C WinAnsi yes yes no 131 0 BLNODK+AdvP4C4E51 Type 1C WinAnsi yes yes no 144 0 BLNOEK+AdvP4C4E74 Type 1C WinAnsi yes yes no 143 0 BLNOFL+AdvTT3f84ef53 Type 1C WinAnsi yes yes no 146 0 BLNOGL+AdvP4C4E46 Type 1C Custom yes yes yes 139 0 BLNOHM+AdvOT46dcae81+22 Type 1C Custom yes yes no 140 0 BLNOJM+AdvTT6780a46b+fb Type 1C Custom yes yes yes 136 0 BLNOJN+AdvOT65f8a23b.I+03 Type 1C Custom yes yes yes 137 0 BLNOLO+AdvOT8608a8d1 Type 1C WinAnsi yes yes no 138 0 BLNOMO+AdvTT6780a46b+22 Type 1C Custom yes yes no 145 0 BLOCKI+Helvetica-Bold Type 1C WinAnsi yes yes no 153 0 BLODAG+Helvetica-BoldOblique Type 1C WinAnsi yes yes no 155 0 BLOHEN+AdvOT65f8a23b.I+fb Type 1C Custom yes yes yes 159 0 BLOLAH+Helvetica Type 1C WinAnsi yes yes no 161 0 BLOONB+Symbol Type 1C WinAnsi yes yes no 160 0 BLOPCA+AdvOT8608a8d1+03 Type 1C Custom yes yes yes 162 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-18 03:07:13 CEST RepresentationInformation: 300281.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-09-17 20:23:40 CEST Size: 824961 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 346 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: A certified plasmid reference material for the standardisation of BCR„–„ABL1 mRNA quantification by real-time quantitative’PCR Destination: title_link Children: Item: Title: Introduction Destination: A1 Item: Title: Materials and methods Destination: A2 Children: Item: Title: Preparation of individual certified plasmid solutions Destination: B1 Item: Title: Digital PCR Destination: B2 Item: Title: Quantitative real-time PCR Destination: B3 Item: Title: Figure 1 Schematic map of the multitarget plasmid pIRMM0099. Destination: fig_bkfigf1 Children: Item: Title: Statistical analysis Destination: B4 Item: Title: Results Destination: A3 Children: Item: Title: Characterisation of copy number concentration Destination: B5 Item: Title: Assessment of homogeneity and stability Destination: B6 Item: Title: Suitability Destination: B7 Item: Title: Table 1 Certified copy number concentrations of double-stranded plasmid DNA and their uncertainties for the six ERM-AD623 dilutions Destination: tab_bktblt1 Item: Title: Discussion Destination: A4 Item: Title: Figure 2 Comparison of measured % BCR-ABL1/ABL1 ratio between laboratory calibrators (Lab) and ERM-AD623 for the cell line dilutions. Destination: fig_bkfigf2 Item: Title: Figure 3 Comparison of measured % BCR-ABL1/ABL1 ratio between laboratory calibrators (Lab) and ERM-AD623 for the aRNA mixtures. Destination: fig_bkfigf3 Item: Title: Table 2 Comparison of results for the cell line dilutions using local calibrators (Lab) and ERM-AD623 Destination: tab_bktblt2 Item: Title: Table 3 Comparison of results for the aRNA mixtures using local calibrators (Lab) and ERM-AD623 Destination: tab_bktblt3 Item: Title: Table 3 Comparison of results for the aRNA mixtures using local calibrators (Lab) and ERM-AD623 Destination: fig_bkfigf4 Item: Title: Figure 5 Estimates of copy numbers of BCR„–„ABL1 and ABL1 transcripts using laboratory calibrators and ERM-AD623 for the aRNA dilutions in the second suitability study. Destination: fig_bkfigf5 Item: Title: Figure 5 Estimates of copy numbers of BCR„–„ABL1 and ABL1 transcripts using laboratory calibrators and ERM-AD623 for the aRNA dilutions in the second suitability study. Destination: A5 Item: Title: Figure 5 Estimates of copy numbers of BCR„–„ABL1 and ABL1 transcripts using laboratory calibrators and ERM-AD623 for the aRNA dilutions in the second suitability study. Destination: A6 Item: Title: ACKNOWLEDGEMENTS Destination: M1 Item: Title: REFERENCES Destination: M4 Info: Title: A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR Author: H White Subject: Leukemia 29, 369 (2015). doi:10.1038/leu.2014.217 Creator: Arbortext Advanced Print Publisher 10.0.1465/W Unicode Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Fri Jan 02 18:00:52 CET 2015 ModDate: Fri Jan 16 16:12:39 CET 2015 ID: 0x0704e514845058c7190fccbcb1f6a91f, 0x6197f7b0400a064d9d8f8e75cd4131b1 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1960 ImageHeight: 1763 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2540 ImageHeight: 1639 BitsPerSample: 8 BitsPerSampleUnit: integer Image: 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