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PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7c53a9eb Children: Item: Title: Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed17bee Children: Item: Title: Animals and experimental conditions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2a33fae0 Item: Title: Preparation and stimulation of head kidney tissue Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@707f7052 Item: Title: Perfusion fatty acid treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11028347 Item: Title: Cortisol measurements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@14899482 Item: Title: Preparation of total RNA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@21588809 Item: Title: Quantitative real-time RT-PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2aae9190 Children: Item: Title: Generation of in vitro-transcribed mRNA for standard curves Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2f333739 Item: Title: Generation of standard curves for stress-related genes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77468bd9 Item: Title: Quantification of transcripts by One-Step TaqMan real-time RT-PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12bb4df8 Item: Title: Sample quantification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4cc77c2e Item: Title: Calculation and statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a7b0070 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@39a054a5 Children: Item: Title: Cortisol released from superfused head kidney Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@71bc1ae4 Item: 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