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2140298 ErrorMessage: Invalid destination object Offset: 2138209 MIMEtype: application/pdf PDFMetadata: Objects: 805 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Abstract Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4032ba08 Children: Item: Title: Background Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42538425 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7908bb27 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2c373b85 Item: Title: Background Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5e24af70 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3a7a1259 Children: Item: Title: DNA methylation profile of myogenic genes harbouring CpG island-promoters during myogenic differentiation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@244aaf0d Item: Title: Myogenic-specific promoters undergo DNA demethylation during muscle-lineage specification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@232a9118 Item: Title: Pax7-induced ES-derived muscle differentiation recapitulates myogenic DNA methylation changes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3f9bd0b1 Item: Title: Impaired myogenic differentiation in Apobec2 knockdown myogenic precursors is associated with reduced DNA demethylation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ea23517 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3b4b8082 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@59e1f537 Item: Title: Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1aa1bfb Children: Item: Title: Skeletal muscle stem cells isolation and culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4712fc97 Item: Title: Cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2f8541f5 Item: Title: Inducible Pax7-ESC-derived myogenic model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@55ad5432 Item: Title: Lentiviral transduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6672ca8f Item: Title: Gene expression analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a50a6d4 Item: Title: Immunofluorescence staining Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44f8e708 Item: Title: Sodium bisulphite conversion, sequencing and pyrosequencing validation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3107e198 Item: Title: Western blot analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7c6bdb4d Item: Title: Availability of data and materials Destination: 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doi:10.1186/s12915-016-0250-9 DNA methylation Cellular identity Myogenesis Pax7-induced ESCs Apobec2 Epimarkers Muscle cell identity requires Pax7-mediated lineage-specific DNA demethylation Elvira Carrió Alessandro Magli Mar Muñoz Miguel A. 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