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Title:          Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
Subject:        Nature Communications 7,  (2016). doi:10.1038/ncomms11084
Author:         Petra Mikolcevic
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        Title: Results
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        Children: 
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          Title: RingoA KO mice are sterile
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6
         Item: 
          Title: RingoA KO spermatocytes arrest at a pachytene-like stage
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f
         Item: 
          Title: Increased non-repaired DNA in RingoA KO spermatocytes
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003
       Item: 
        Title: Figure™1RingoA KO males are sterile.(a) RingoA-deficient mice are indistinguishable from their littermates. (b) RingoA immunoprecipitates from WT and KO testis lysates were immunoblotted for RingoA. The asterisk indicates IgGs. (c) RingoA KO testes are ab
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        Children: 
         Item: 
          Title: RingoA co-localizes with Cdk2 at telomeres
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb
         Item: 
          Title: RingoA loss negatively affects meiotic telomere function
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431
       Item: 
        Title: Figure™2RingoA KO spermatocytes arrest in a pachytene-like stage with aberrant synapsis.(a) WT and KO spread spermatocytes were immunolabelled for Sycp3 (red) and Sycp1 (green). KO spermatocytes presented non-homologous pairing and partner switching (arro
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6
       Item: 
        Title: Figure™3RingoA KO spermatocytes show non-repaired DSBs and lack of crossing-overs.(a) WT and KO spread spermatocytes were immunolabelled for Sycp3 (red) and gammaH2AX (green). gammaH2AX marked DSBs in leptotene WT and KO spermatocytes. In pachytene WT spe
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a
        Children: 
         Item: 
          Title: Disrupted telomere architecture in the absence of RingoA
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71
       Item: 
        Title: Figure™4RingoA is required for Cdk2 localization and co-localizes with Cdk2 at telomeres in prophase I spermatocytes.(a) WT and KO spread spermatocytes were immunolabelled for Sycp3 (red) and either RingoA (green) or Cdk2 (green). In WT pachytene spermato
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2
       Item: 
        Title: Discussion
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9
       Item: 
        Title: Figure™5RingoA interacts with Cdk2 and regulates Cdk2 kinase activity in testis.(a) Immunoprecipitates from WT testis lysates using RingoA or Cdk2 antibodies and control IgGs were analysed by immunoblotting for RingoA. The arrowhead indicates RingoA and t
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f
       Item: 
        Title: Figure™6RingoA loss negatively affects meiotic telomere function.(a) WT and KO spread spermatocytes were immunolabelled for Sycp3 (red) and Trf1 (green). In pachytene-like KO spermatocytes, Trf1 can be seen along the SC threads, instead of at the ends, in
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78
       Item: 
        Title: Methods
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee
        Children: 
         Item: 
          Title: Generation and analysis of RingoA KO mice
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f
         Item: 
          Title: Histology and TUNEL staining
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1
       Item: 
        Title: Figure™7RingoA regulates Sun1 telomeric localization.(a) WT and KO spread spermatocytes were immunolabelled for Sycp3 (red) and Terb1 (green). Terb1 signals at telomeres were unperturbed in RingoA KO spermatocytes. (b) WT and KO spread spermatocytes were 
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d
       Item: 
        Title: Figure™8Meiotic telomere architecture is disrupted in the absence of RingoA.WT and KO spread spermatocytes were immunolabeled for Sycp3 (red) and either H3K9-3me or H3K4-3me (green in a,b). (a) KO spermatocytes showed weakened H3K9-3me staining concentrat
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89
        Children: 
         Item: 
          Title: Meiotic spreads and squashes
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9
         Item: 
          Title: Immunostaining and telomere FISH
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e
         Item: 
          Title: Microscopy
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b
         Item: 
          Title: Transmission electron microscopy
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b
         Item: 
          Title: Statistical analysis
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31
         Item: 
          Title: Immunoprecipitation and immunoblotting
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107
         Item: 
          Title: Recombinant protein production
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e
         Item: 
          Title: Kinase assays
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7
         Item: 
          Title: Real-time quantitative PCR
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e
       Item: 
        Title: MorganD. O.Cyclin-dependent kinases: engines, clocks and microprocessorsAnnu. Rev. Cell Dev. Biol.132612911997NebredaA. R.CDK activation by non-cyclin proteinsCurr. Opin. Cell Biol.181921982006FerbyI.BlazquezM.PalmerA.EritjaR.NebredaA. R.A novel p34(cdc2)
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247
       Item: 
        Title: We are grateful to Elisabeth Llonch and Lorena Ramírez, the Advanced Digital Microscopy and Mouse Mutant facilities of IRB Barcelona, and the Transmission Electron Microscopy Facility of the PCB for excellent technical assistance. We thank Marko Marjanovi
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9
       Item: 
        Title: ACKNOWLEDGEMENTS
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       Item: 
        Title: Author contributions
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a
       Item: 
        Title: Additional information
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30
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    Title: Essential role of the Cdk2 activator RingoA in meiotic telomere tethering to the nuclear envelope
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