a41112725b41eb0792c981e9f3c6fd88 pmc_27456200.pdf fe2a586dd523d1c75207b677c2e73ab332932b1e pmc_27456200.pdf 92e47ce8e64636bec1b5836b39726244edcc426a0ea154438bdf473ad199cb1d pmc_27456200.pdf Title: Single cell–resolution western blotting Subject: Nature Protocols 11, 1508 (2016). doi:10.1038/nprot.2016.089 Author: Chi-Chih Kang Creator: Adobe InDesign CS2 (4.0) Producer: Adobe PDF Library 7.0 CreationDate: Tue Jul 19 11:51:36 2016 CEST ModDate: Wed Jul 20 10:22:01 2016 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 876942 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IDAEIL+AdvOTea1a7398 Type 1C Custom yes yes yes 422 0 IDAEIM+AdvOTcb88df00 Type 1C Custom yes yes yes 430 0 IDAENL+Arial-BoldMT Type 1C WinAnsi yes yes no 431 0 IDAEPL+AdvOTf23bb480 Type 1C Custom yes yes yes 432 0 IDAFEK+AdvTTda6f6cb8.B Type 1C Custom yes yes yes 442 0 IDAFMO+AdvPi1 Type 1C WinAnsi yes yes no 178 0 IDAFLJ+AdvOT125c650c Type 1C Custom yes yes yes 175 0 IDAFLK+AdvOT1ef757c0 Type 1C Custom yes yes yes 158 0 IDAFLL+AdvOT7d6df7ab.I Type 1C Custom yes yes yes 159 0 IDAFML+AdvPi2 Type 1C WinAnsi yes yes no 169 0 IDAFMM+AdvP4C4E74 Type 1C Custom yes yes yes 170 0 IDAFMN+AdvP4C4E46 Type 1C Custom yes yes yes 183 0 IDAGBN+AdvOTb65e897d.B Type 1C Custom yes yes yes 203 0 IDBDIM+Helvetica Type 1C WinAnsi yes yes no 204 0 IDBDJN+Helvetica-Oblique Type 1C WinAnsi yes yes no 186 0 IDBDJO+Helvetica-Bold Type 1C WinAnsi yes yes no 195 0 IDBDJP+Symbol Type 1C Custom yes yes yes 212 0 IDBEPH+AdvOT9bd21c25.I Type 1C Custom yes yes yes 207 0 IDBFAI+AdvEls-ent7 Type 1C WinAnsi yes yes no 208 0 IDBFFH+AdvOT65f8a23b.I Type 1C Custom yes yes yes 219 0 IDBGDD+AdvOTe75a9404.I Type 1C Custom yes yes yes 220 0 IDBGHC+AdvEls-Ent1 Type 1C WinAnsi yes yes no 223 0 IDBGKC+AdvEls-ent4 Type 1C WinAnsi yes yes no 230 0 IDBGKD+AdvEls-ent2 Type 1C WinAnsi yes yes no 225 0 IDBPLM+AdvGreek_B Type 1C WinAnsi yes yes no 233 0 IDBPNN+AdvItcSymbol-M Type 1C WinAnsi yes yes no 236 0 IDCPFO+Advsymbol Type 1C Custom yes yes yes 237 0 IDCPLN+AdvP4C4E51 Type 1C Custom yes yes yes 243 0 IDDANH+AdvEls-ent3 Type 1C WinAnsi yes yes no 247 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-16 03:00:58 CET RepresentationInformation: pmc_27456200.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2018-02-06 08:56:07 CET Size: 876942 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 465 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: title_link Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Children: Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Children: Item: Title: Molecular synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Figure™1Schematic illustrations of the junctions.Schematic representations of the junctions of AuTS-SC10PTMRsolNRsolsolGaOxcondsolEGaIn and the corresponding energy level diagrams. The top-electrodes (a) are liquid metal GaOxcondsolEGaIn (where EGaIn is t Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Children: Item: Title: Outline placeholder Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Children: Item: Title: SAM structural characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Figure™2Synthetic route to radicals HSCnPTMR and non-radicals HSCnPTMNR.DBU, 1, 8-diazabicycloundec-7-ene; tBuOK, potasium tert-butoxide; DMSO, dimtehylsulfoxide; DCM, dichloromethane; ET3N, triethylamine; SO3Py, sulfur trioxide pyridine; Bu4NOH, tetrabut Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Children: Item: Title: Outline placeholder Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Children: Item: Title: SAM electronic structure characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Transport through the SAMs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Figure™3Electronic structure of the PTMRsolNR SAMs on AuTS.(a) Ultraviolet photoemission spectroscopy (UPS) and (b) C K- edge X- ray absorption fine structure (NEXAFS) spectra of SAMs derived from HSCnPTMR with n=8, 10, 12 and HSC12PTMNR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Table 1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: Figure™4Electrical characteristics of the tunnelling junctions at room temperature.(a,c,e) Plots of the ltlogmidJmidgtG against the applied bias for radical (red) and non-radical (black) junctions and (b,d,f) the histograms of logmidJmid measured at -1.0t Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Figure™5Temperature dependence measurements.(a) Semi-log plots of the average J(V) curves measured over the temperature range of 210-340thinspK at intervals of 10thinspK for AuTS-SCnPTMR SAMs with n=8, 10 and 12 and (b) Arrhenius plots of the average J at Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Figure™6General tunnelling equation fit.The average values of J (Athinspcm-2) at -1.0thinspV as a function of carbon number (n) for AuTS-SCnPTMRsolNRsolsolGaOxcondsolEGaIn junctions. The dashed lines represented the fits to the general tunnelling equation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Children: Item: Title: Synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: Fabrication of template-stripped bottom-electrode Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: Formation of the SAMs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Electrochemical characterization of the SAMs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: Fabrication of PDMS top-electrodes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: Fabrication of AuTS-SCnPTMRsolNRsolsolGaOxcondsolEGaIn junctions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: Stability measurement of AuTS-SC8PTMRsolNRsolsolGaOxcondsolEGaIn junctions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: Photoelectron spectroscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: Determination of the surface coverage of the SAMs from the S 2p spectra Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: Determination of the thickness of the SAMs from the S 2p spectra Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: Calculation of LUMO energy level from NEXAFS spectra Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: Temperature-dependent measurement of a junction with a SAM of NR8 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: LAD fitting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: Data availability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: AkkermanH. B.de BoerB.Electrical conduction through single molecules and self-assembled monolayersJ. Phys. Condens. Matter200130012008AviramA.RatnerM.Molecular rectifiersChem. Phys. Lett.292772831974CuiX. D.Making electrical contacts to molecular monolaye Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: The National Research Foundation, Prime MinisterCloseCurlyQuotes Office, Singapore under its Medium sized centre programme, and for the NRF fellowship to C.A.N., award No. NRF-RF 2010-03, is kindly acknowledged for supporting this research. 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Nonsymbolic FontBBox: 0, -208, 1083, 739 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: IDAFEK+AdvTTda6f6cb8.B FontSubset: true FirstChar: 48 LastChar: 57 FontDescriptor: FontName: IDAFEK+AdvTTda6f6cb8.B Flags: Serif, Symbolic FontBBox: -93, -218, 1000, 875 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: IDBDJN+Helvetica-Oblique FontSubset: true FirstChar: 32 LastChar: 120 FontDescriptor: FontName: IDBDJN+Helvetica-Oblique Flags: Nonsymbolic, Italic FontBBox: -170, -225, 1116, 931 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf doi:10.1038/nprot.2016.089 Chi-Chih Kang Kevin A Yamauchi Julea Vlassakis Elly Sinkala Todd A Duncombe Amy E Herr Nature Protocols 11, 1508 (2016). doi:10.1038/nprot.2016.089 Nature Publishing Group © 2016 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. Single cell–resolution western blotting Adobe PDF Library 7.0 © 2016 Nature Publishing Group, a division of Macmillan Publishers Limited. 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