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Title:          Mucosal immunoglobulins at respiratory surfaces mark an ancient association that predates the emergence of tetrapods
Subject:        Nature Communications 7,  (2016). doi:10.1038/ncomms10728
Author:         Zhen Xu
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      Title: title_link
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       Item: 
        Title: Results
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4
        Children: 
         Item: 
          Title: Immunoglobulins and B cells in the gill of trout
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034
         Item: 
          Title: IgT constitutes the main Ig isotype coating the gill microbiota
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6
       Item: 
        Title: Figure™1Protein characterization of gill mucus immunoglobulins and identification of B cells in the gill of trout.(a) Fractionation of gill mucus (sim0.5thinspml) by gel filtration (upper) followed by immunoblot analysis of the fractions with anti-trout I
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f
        Children: 
         Item: 
          Title: Pathogen-specific immunoglobulin responses in the gill
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003
       Item: 
        Title: Figure™2Trout gill bacteria are predominantly coated with IgT.(a) Representative flow cytometry histograms showing the staining of gill bacteria with IgT, IgM and IgD. Bacteria were stained with anti-trout IgT (green line), anti-trout IgM (red line) or an
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        Children: 
         Item: 
          Title: Local proliferative and immunoglobulin responses
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb
         Item: 
          Title: pIgR in the gill of trout
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431
       Item: 
        Title: Figure™3Parasites are mainly coated by IgT in the gill of infected trout.Four different microscope images (a-d) of slides showing immunofluorescence staining of Ich parasites in gill cryosections from trout infected with Ich after 25 days (n=6). From left
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6
       Item: 
        Title: Discussion
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a
       Item: 
        Title: Figure™4Increases in IgT+ B cells in the gill of trout infected with Ich.(a-c) DIC images of immunofluorescence staining on trout gill cryosections from uninfected fish (a), 25 days infected fish (b) and survivor fish (c, upper), stained for IgT (green) a
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71
       Item: 
        Title: Figure™5Immunoglobulin responses in the gill mucus and serum from infected and survivor fish.(a) Western blot analysis of IgT-, IgM- and IgD-specific binding to Ich in gill mucus (dilution 1:2) from infected and survivor fish. (b,c) IgT-, IgM- and IgD-spe
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2
       Item: 
        Title: Figure™6Proliferative responses of IgT+ and IgM+ B cells in the gill of fish that survived Ich infection.(a,b) Representative flow cytometry dot plot showing proliferation of IgT+ B cells (a) and IgM+ B cells (b) in gill leucocytes of control and survivor
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9
       Item: 
        Title: Figure™7Local IgT-, IgM- and IgD-specific responses in gill explants of survivor fish.Gill, head kidney and spleen explants (sim50thinspmg each) from control and survivor fish were cultured for 7 days. (a-c) Western blot analysis of IgT-, IgM- and IgD-spe
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f
       Item: 
        Title: Figure™8Trout pIgR associates with gill sIgs.(a) SDS-PAGE under reducing conditions of trout serum and gill mucus (sim5thinspmgrg each), followed by immunoblot analysis using anti-trout pIgR antibody. (b) Co-immunoprecipitation (CoIP) of gill mucus with a
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78
       Item: 
        Title: Methods
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee
        Children: 
         Item: 
          Title: Fish maintenance
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f
         Item: 
          Title: Ich parasite isolation and infection
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1
         Item: 
          Title: F. columnare infection
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d
         Item: 
          Title: Collection of gill mucus and bacteria
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89
       Item: 
        Title: Figure™9Proposed model of local IgT and IgT+ B-cell induction in the gills.(a) Scheme of a typical teleost gill filament displaying the primary (PL) and secondary (SL) lamellae. (b) Induction of local IgT responses in the gill based on our findings. On Ic
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9
        Children: 
         Item: 
          Title: Purification and LC-MSsolMS analysis of gill mucus IgT
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e
         Item: 
          Title: Isolation of leucocytes from the gill and nasal cavities
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b
         Item: 
          Title: SDS-PAGE and western blot
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b
         Item: 
          Title: Flow cytometry
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31
         Item: 
          Title: Gel filtration
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107
         Item: 
          Title: Immunofluorescence microscopy studies
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e
         Item: 
          Title: Intracellular staining
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7
         Item: 
          Title: Proliferation of B cells in the gill of trout
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e
         Item: 
          Title: Tissue explants culture
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247
         Item: 
          Title: Binding of trout immunoglobulins to Ich
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9
         Item: 
          Title: Co-immunoprecipitation studies
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36
         Item: 
          Title: Statistical analysis
          Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a
       Item: 
        Title: MainaJ. N.Structure, function and evolution of the gas exchangers: comparative perspectivesJ. Anat.2012813042002GloverC. N.BuckingC.WoodC. M.The skin of fish as a transport epithelium: a reviewJ. Comp. Physiol. B1838778912013AquilinoC.CastroR.FischerU.Taf
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30
       Item: 
        Title: We would like to thank the Morphology Core of the Center for Molecular Studies in Digestive and Liver Diseases (the University of Pennsylvania), especially Adam Bedenbaugh, for the help and advice provided for the immunohistochemistry work. We would also 
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       Item: 
        Title: ACKNOWLEDGEMENTS
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       Item: 
        Title: Author contributions
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6
       Item: 
        Title: Additional information
        Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e
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    Title: Mucosal immunoglobulins at respiratory surfaces mark an ancient association that predates the emergence of tetrapods
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