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Title:          Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
Subject:        Microbial Cell Factories, doi:10.1186/s12934-016-0457-z
Keywords:       Protein engineering; Recombinant proteins; Nanoparticles; Nanomedicine; Biomaterials; Biodistribution; E. coli; Endotoxin-free strains
Author:         Fabián Rueda
Creator:        Springer
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     Item: 
      Title: Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
      Children: 
       Item: 
        Title: Abstract 
        Children: 
         Item: 
          Title: Background: 
         Item: 
          Title: Results: 
         Item: 
          Title: Discussion: 
       Item: 
        Title: Background
       Item: 
        Title: Methods
        Children: 
         Item: 
          Title: Strains, plasmids and media
         Item: 
          Title: Competent cells and transformation
         Item: 
          Title: Protein production and purification
         Item: 
          Title: Western blot analysis
         Item: 
          Title: Fluorescence intensity
         Item: 
          Title: Particle size and zeta potential
         Item: 
          Title: Mass spectrometry (MS) analysis
         Item: 
          Title: Circular dichroism (CD)
         Item: 
          Title: Electron microscopy
         Item: 
          Title: Analysis of cell internalization
         Item: 
          Title: Confocal microscopy
         Item: 
          Title: Determination of in vivo biodistribution
         Item: 
          Title: Histological and immunohistochemical evaluation
         Item: 
          Title: Quantitative real-time PCR
       Item: 
        Title: Results and discussion
       Item: 
        Title: Conclusions
       Item: 
        Title: Authors’ contributions
       Item: 
        Title: References
   Info: 
    Title: Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
    Author: Fabián Rueda
    Subject: Microbial Cell Factories, doi:10.1186/s12934-016-0457-z
    Keywords: Protein engineering; Recombinant proteins; Nanoparticles; Nanomedicine; Biomaterials; Biodistribution; E. coli; Endotoxin-free strains
    Creator: Springer
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