07be523eb076266c19a4c5f88a8451f5 pmc_5164931.pdf 11852b91b8ae63578ffc726814f828d0f92cc453 pmc_5164931.pdf 5469be83e5abc230883d7e44e6607e2504935ff6b676e8d99ad27ad9008a57c7 pmc_5164931.pdf Title: Trans-presentation of interleukin-6 by dendritic cells is required for priming pathogenic TH17 cells Author: Sylvia Heink, Nir Yogev, Christoph Garbers, Marina Herwerth, Lilian Aly, Christiane Gasperi, Veronika Husterer, Andrew L. Croxford, Katja Möller-Hackbarth, Harald S. Bartsch, Karl Sotlar, Stefan Krebs, Tommy Regen, Helmut Blum, Bernhard Hemmer, Thomas Misgeld, Thomas F. Wunderlich, Juan Hidalgo, Mohamed Oukka, Stefan Rose-John, Marc Schmidt-Supprian, Ari Waisman, Thomas Korn Creator: AH XSL Formatter V6.3 R1 for Linux64 : 6.3.1.23495 (2016/02/03 14:39JST) Producer: Antenna House PDF Output Library 6.3.752 (Linux64) CreationDate: Tue Dec 20 21:51:26 2016 CET ModDate: Tue Dec 20 21:51:26 2016 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 34 Encrypted: no Page size: 612 x 792 pts (letter) Page rot: 0 File size: 3042332 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- Times-Roman Type 1 Custom no no yes 11 0 Helvetica-Bold Type 1 Custom no no yes 13 0 Helvetica Type 1 Custom no no yes 17 0 Times-Bold Type 1 Standard no no no 80 0 Helvetica-BoldOblique Type 1 Standard no no no 86 0 HMOLCO+Code2000 CID TrueType Identity-H yes yes yes 102 0 Times-BoldItalic Type 1 Custom no no yes 771 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-16 02:39:06 CET RepresentationInformation: pmc_5164931.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-10-12 02:17:08 CEST Size: 3042332 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul ID: PDF-HUL-128 InfoMessage: Outlines contain recursive references. MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 0 FreeObjects: 104 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Abstract Item: Title: Results Children: Item: Title: DCs are the relevant source of IL-6 for priming pathogenic TH17 cells Item: Title: DC derived IL-6 is sensed by interacting T cells in a specific manner Item: Title: IL-6 cluster signaling is an efficient signaling mode Item: Title: DCs present IL-6 in trans and induce IL-6 cluster signaling Item: Title: IL-6 and IL-6R͜㈶㄀ 挀漀ⴀ氀漀挀愀氀椀稀攀 椀渀 䐀䌀猩ਯ䌠嬠〠〠〠崠⽁‼㰠⽓ 䝯呯 䐠㤹㌠〠删㸾ਯ偡牥湴‹㠲‰⁒ 偲敶‹㤰‰⁒ 乥硴‹㤴‰⁒‾㸊敮摯扪ਹ㤵‰扪ਜ਼″ㄸ‰⁒ 塙娠湵汬″㠲畬氠崊敮摯扪ਹ㤴‰扪਼㰠⽔楴汥
䥌ⴶ⁣汵獴敲⁳楧湡汩湧⁤畲楮朠慮瑩来渭獰散楦楣⁔⁣敬氠灲業楮朠楮⁶楶漩ਯ䌠嬠〠〠〠崠⽁‼㰠⽓ 䝯呯 䐠㤹㔠〠删㸾ਯ偡牥湴‹㠲‰⁒ 偲敶‹㤲‰⁒ 乥硴‹㤶‰⁒‾㸊敮摯扪ਹ㤷‰扪ਜ਼″㔱‰⁒ 塙娠湵汬″㘰畬氠崊敮摯扪ਹ㤶‰扪਼㰠⽔楴汥
偡瑨潧敮楣⁔䠱㜠捥汬猠慲攠摥灥湤敮琠潮⁉䰭㘠捬畳瑥爠獩杮慬楮朩ਯ䌠嬠〠〠〠崠⽁‼㰠⽓ 䝯呯 䐠㤹㜠〠删㸾ਯ偡牥湴‹㠲‰⁒ 偲敶‹㤴‰⁒‾㸊敮摯扪ਹ㠳‰扪ਜ਼‱㐱‰⁒ 塙娠湵汬‵㈸畬氠崊敮摯扪ਹ㠲‰扪਼㰠⽆楲獴‹㠴‰⁒ 䱡獴‹㤶‰⁒ 呩瑬攠⡒敳畬瑳 Item: Title: Discussion Item: Title: Online methods Children: Item: Title: Generation of Il6RD mice Item: Title: Mice Item: Title: Induction of EAE Item: Title: Preparation of CNS mononuclear cells Item: Title: Preparation of naïve T cells and differentiation of T helper cell subsets Item: Title: Isolation of DCs Item: Title: Generation and culture of BMDCs Item: Title: BMDC / T cell co-culture Item: Title: Flow cytometry and intracellular cytokine staining Item: Title: Detection of antigen specific T cells ex vivo Item: Title: ELISA Item: Title: Quantitative PCR analysis Item: Title: RNA Seq Item: Title: Immunofluorescence, proximity ligation assay (PLA), and confocal microscopy Item: Title: Statistical analysis Item: Title: Data availability statement Item: Title: References Item: Title: Figure 1 Item: Title: Figure 2 Item: Title: Figure 3 Item: Title: Figure 4 Item: Title: Figure 5 Item: Title: Figure 6 Item: Title: Figure 7 Item: Title: Figure 8 Item: Title: Discussion Item: Title: Online methods Children: Item: Title: References Item: Title: Figure 1 Item: Title: Figure 2 Item: Title: Figure 3 Item: Title: Figure 4 Item: Title: Figure 5 Item: Title: Figure 6 Item: Title: Figure 7 Item: Title: Figure 8 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1688 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1768 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 1422 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 2013 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1304 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2064 ImageHeight: 2099 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1896 ImageHeight: 2099 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1528 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type0: Font: BaseFont: HMOLCO+Code2000 Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: Times-Bold Font: BaseFont: Helvetica FirstChar: 32 LastChar: 182 FontDescriptor: FontName: Helvetica Flags: Nonsymbolic FontBBox: -166, -225, 1000, 931 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Times-BoldItalic FirstChar: 32 LastChar: 128 FontDescriptor: FontName: Times-BoldItalic Flags: Nonsymbolic, Italic FontBBox: -200, -218, 996, 921 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Helvetica-BoldOblique Font: BaseFont: Times-Roman FirstChar: 32 LastChar: 182 FontDescriptor: FontName: Times-Roman Flags: Nonsymbolic FontBBox: -168, -218, 1000, 898 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Helvetica-Bold FirstChar: 32 LastChar: 129 FontDescriptor: FontName: Helvetica-Bold Flags: Nonsymbolic FontBBox: -170, -228, 1003, 962 EncodingDictionary: Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: HMOLCO+Code2000 CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: HMOLCO+Code2000 Flags: Symbolic FontBBox: -1111, -295, 2107, 977 FontFile2: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 230, 202, 450, 210 LastModified: D:20161220155126-05'00' Page: Sequence: 2 Page: Sequence: 3 Page: Sequence: 4 Page: Sequence: 5 Page: Sequence: 6 Page: Sequence: 7 Page: Sequence: 8 Page: Sequence: 9 Page: Sequence: 10 Page: Sequence: 11 Page: Sequence: 12 Page: Sequence: 13 Page: Sequence: 14 Page: Sequence: 15 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 250, 678, 360, 688 LastModified: D:20161220155127-05'00' Page: Sequence: 17 Page: Sequence: 18 Page: Sequence: 19 Page: Sequence: 20 Page: Sequence: 21 Page: Sequence: 22 Page: Sequence: 23 Page: Sequence: 24 Page: Sequence: 25 Page: Sequence: 26 Page: Sequence: 27 Page: Sequence: 28 Page: Sequence: 29 Page: Sequence: 30 Page: Sequence: 31 Page: Sequence: 32 Page: Sequence: 33 Page: Sequence: 34 Checksum: 0986c816 Type: CRC32 Checksum: 07be523eb076266c19a4c5f88a8451f5 Type: MD5 Checksum: 11852b91b8ae63578ffc726814f828d0f92cc453 Type: SHA-1