9b67b72eb0f60cbbd3e23c2e3ccb70fa IntJouMolSci_a2017v18n1a65iENG.pdf d7e72c7080d5e9cd69c19f7ee9381b56c15c4432 IntJouMolSci_a2017v18n1a65iENG.pdf 51dd8f49ae04cd123f475e4df88d3ca0ee9531b315552d1b3356f357a5b9e738 IntJouMolSci_a2017v18n1a65iENG.pdf Title: Preferential Enhancement of Sensory and Motor Axon Regeneration by Combining Extracellular Matrix Components with Neurotrophic Factors Subject: After peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable environment to promote axonal growth. Here, we assessed in vitro and in vivo the selective effects of combining different ECM components with NTFs on motor and sensory axons regeneration and target reinnervation. Organotypic cultures with collagen, laminin and nerve growth factor (NGF)/neurotrophin-3 (NT3) or collagen, fibronectin and brain-derived neurotrophic factor (BDNF) selectively enhanced sensory neurite outgrowth of DRG neurons and motor neurite outgrowth from spinal cord slices respectively. For in vivo studies, the rat sciatic nerve was transected and repaired with a silicone tube filled with a collagen and laminin matrix with NGF/NT3 encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres (MP) (LM + MP.NGF/NT3), or a collagen and fibronectin matrix with BDNF in PLGA MPs (FN + MP.BDNF). Retrograde labeling and functional tests showed that LM + MP.NGF/NT3 increased the number of regenerated sensory neurons and improved sensory functional recovery, whereas FN + MP.BDNF preferentially increased regenerated motoneurons and enhanced motor functional recovery. Therefore, combination of ECM molecules with NTFs may be a good approach to selectively enhance motor and sensory axons regeneration and promote appropriate target reinnervation. Keywords: neurotrophic factors; BDNF; NGF; NT3; extracellular matrix; motor axons; sensory axons; nerve regeneration; reinnervation Author: Daniel Santos, Francisco González-Pérez, Guido Giudetti, Silvestro Micera, Esther Udina, Jaume Del Valle and Xavier Navarro Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Thu Dec 29 18:04:01 2016 ModDate: Thu Dec 29 18:04:01 2016 Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 7481930 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TKOSIR+URWPalladioL-Ital Type 1 Custom yes yes no 91 0 EHUTTP+URWPalladioL-Bold Type 1 Custom yes yes no 92 0 ENZYBP+URWPalladioL-Roma Type 1 Custom yes yes no 93 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 97 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 98 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 154 0 OIPCAC+PalatinoLinotype TrueType WinAnsi yes yes no 235 0 OIPCED+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 236 0 OIPCFE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 237 0 OIPDKN+Calibri TrueType WinAnsi yes yes no 238 0 OIPCAC+PalatinoLinotype TrueType WinAnsi yes yes no 698 0 OIPCED+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 699 0 OIPCFE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 700 0 OIPDKN+Calibri TrueType WinAnsi yes yes no 701 0 OIPCAC+PalatinoLinotype TrueType WinAnsi yes yes no 728 0 OIPCED+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 729 0 OIPCFE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 730 0 OIPDKN+Calibri TrueType WinAnsi yes yes no 731 0 OIPCAC+PalatinoLinotype TrueType WinAnsi yes yes no 760 0 OIPCED+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 761 0 OIPCFE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 762 0 OIPDKN+Calibri TrueType WinAnsi yes yes no 763 0 OIPCAC+PalatinoLinotype TrueType WinAnsi yes yes no 791 0 OIPCED+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 792 0 OIPCFE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 793 0 OIPDKN+Calibri TrueType WinAnsi yes yes no 794 0 SQADDB+EURM10 Type 1 Builtin yes yes no 840 0 java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary at edu.harvard.hul.ois.jhove.module.PdfModule.readDocCatalogDict(PdfModule.java:1284) at edu.harvard.hul.ois.jhove.module.PdfModule.parse(PdfModule.java:525) at edu.harvard.hul.ois.jhove.JhoveBase.processFile(JhoveBase.java:825) at edu.harvard.hul.ois.jhove.JhoveBase.process(JhoveBase.java:614) at edu.harvard.hul.ois.jhove.JhoveBase.dispatch(JhoveBase.java:465) at Jhove.main(Jhove.java:296) Jhove (Rel. 1.6, 2011-01-04) Date: 2017-01-31 02:42:17 CET RepresentationInformation: IntJouMolSci_a2017v18n1a65iENG.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2017-01-27 13:34:25 CET Size: 7481930 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: 1f959d6c Type: CRC32 Checksum: 9b67b72eb0f60cbbd3e23c2e3ccb70fa Type: MD5 Checksum: d7e72c7080d5e9cd69c19f7ee9381b56c15c4432 Type: SHA-1