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MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: 1. Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Children: Item: Title: 1. Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 2. Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Children: Item: Title: 2.1. Semen samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 2.2. Sperm cryopreservation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 2.3. Evaluation of sperm concentration and morphology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 2.4. Evaluation of plasma membrane integrity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 2.5. Evaluation of sperm motility Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 2.6. Statistical analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Children: Item: Title: 2.6.1. Classification of ejaculates into groups of good (GFE) and poor freezability (PFE) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: 2.6.2. Determination of sperm subpopulations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: 3. Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Children: Item: Title: 3.1. Classification of boar ejaculates Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: 3.2. Effects of GSH on sperm membrane integrity and motility in GFE and PFE Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: 3.3. Principal component analysis and determination of sperm subpopulations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: 3.4. Effects of GSH on distribution of sperm subpopulations in GFE and PFE Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: 4. Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Item: Title: Acknowledgements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e Item: Title: Appendix A. Supplementary data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@cb0ed20 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8e24743 Item: Title: 2. Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74a10858 Children: Item: Title: 3. Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@23fe1d71 Children: Item: Title: 4. Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@28ac3dc3 Item: Title: Acknowledgements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@32eebfca Item: Title: Appendix A. 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CMJBEJ+AdvOT863180fb+20 Flags: Nonsymbolic FontBBox: 9, -208, 947, 781 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf doi:10.1016/j.cryobiol.2017.07.002 The addition of reduced glutathione to cryopreservation media induces changes in the structure of motile subpopulations of frozen-thawed boar sperm Efrén Estrada Maria M. Rivera del Álamo Joan E. Rodríguez-Gil Marc Yeste Pigs Reduced glutathione GSH Spermatozoa Cryopreservation Motile sperm subpopulations Cryobiology, 78 (2017) 56-64. doi:10.1016/j.cryobiol.2017.07.002 Elsevier Ltd journal Cryobiology © 2017 Elsevier Inc. 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Subtype: Link Rect: 175, 50, 209, 56 Annotation: Subtype: Link Rect: 212, 50, 232, 56 Annotation: Subtype: Link Rect: 235, 50, 293, 56 Annotation: Subtype: Link Rect: 327, 720, 562, 727 Annotation: Subtype: Link Rect: 327, 712, 562, 719 Annotation: Subtype: Link Rect: 327, 704, 361, 711 Annotation: Subtype: Link Rect: 366, 704, 377, 711 Annotation: Subtype: Link Rect: 327, 696, 562, 703 Annotation: Subtype: Link Rect: 327, 688, 510, 695 Annotation: Subtype: Link Rect: 515, 688, 526, 695 Annotation: Subtype: Link Rect: 327, 680, 562, 687 Annotation: Subtype: Link Rect: 327, 672, 562, 679 Annotation: Subtype: Link Rect: 327, 664, 562, 671 Annotation: Subtype: Link Rect: 327, 656, 339, 663 Annotation: Subtype: Link Rect: 344, 656, 355, 663 Annotation: Subtype: Link Rect: 327, 648, 562, 655 Annotation: Subtype: Link Rect: 327, 640, 339, 647 Annotation: Subtype: Link Rect: 344, 640, 355, 647 Annotation: Subtype: Link Rect: 327, 632, 562, 639 Annotation: Subtype: Link Rect: 327, 625, 562, 631 Annotation: Subtype: Link Rect: 327, 617, 495, 623 Annotation: Subtype: Link Rect: 500, 617, 512, 623 Annotation: Subtype: Link Rect: 327, 609, 562, 615 Annotation: Subtype: Link Rect: 327, 601, 562, 607 Annotation: Subtype: Link Rect: 327, 593, 361, 599 Annotation: Subtype: Link Rect: 366, 593, 377, 599 Annotation: Subtype: Link Rect: 366, 561, 473, 567 Annotation: Subtype: Link Rect: 327, 553, 541, 559 Annotation: Subtype: Link Rect: 545, 553, 562, 559 Annotation: Subtype: Link Rect: 327, 545, 562, 551 Annotation: Subtype: Link Rect: 327, 537, 562, 543 Annotation: Subtype: Link Rect: 327, 529, 361, 535 Annotation: Subtype: Link Rect: 363, 529, 562, 535 Annotation: Subtype: Link Rect: 327, 521, 342, 527 Annotation: Subtype: Link Rect: 347, 521, 363, 527 Annotation: Subtype: Link Rect: 327, 513, 446, 519 Annotation: Subtype: Link Rect: 449, 513, 562, 519 Annotation: Subtype: Link Rect: 327, 505, 368, 511 Annotation: Subtype: Link Rect: 372, 505, 389, 511 Annotation: Subtype: Link Rect: 392, 505, 562, 511 Annotation: Subtype: Link Rect: 327, 497, 562, 503 Annotation: Subtype: Link Rect: 327, 489, 529, 495 Annotation: Subtype: Link Rect: 534, 489, 545, 495 Annotation: Subtype: Link Rect: 327, 481, 343, 487 Annotation: Subtype: Link Rect: 347, 481, 433, 487 Annotation: Subtype: Link Rect: 437, 481, 523, 487 Annotation: Subtype: Link Rect: 527, 481, 562, 487 Annotation: Subtype: Link Rect: 327, 473, 562, 479 Annotation: Subtype: Link Rect: 327, 465, 562, 472 Annotation: Subtype: Link Rect: 327, 457, 447, 464 Annotation: Subtype: Link Rect: 452, 457, 468, 464 Annotation: Subtype: Link Rect: 327, 449, 503, 456 Annotation: Subtype: Link Rect: 508, 449, 519, 456 Annotation: Subtype: Link Rect: 524, 449, 562, 456 Annotation: Subtype: Link Rect: 327, 441, 364, 448 Annotation: Subtype: Link Rect: 370, 441, 382, 448 Annotation: Subtype: Link Rect: 387, 441, 562, 448 Annotation: Subtype: Link Rect: 327, 433, 562, 440 Annotation: Subtype: Link Rect: 327, 425, 522, 432 Annotation: Subtype: Link Rect: 527, 425, 542, 432 Annotation: Subtype: Link Rect: 327, 417, 523, 424 Annotation: Subtype: Link Rect: 528, 417, 540, 424 Annotation: Subtype: Link Rect: 327, 409, 347, 416 Annotation: Subtype: Link Rect: 351, 409, 499, 416 Annotation: Subtype: Link Rect: 503, 409, 562, 416 Annotation: Subtype: Link Rect: 327, 401, 562, 408 Annotation: Subtype: Link Rect: 327, 393, 562, 400 Annotation: Subtype: Link Rect: 327, 385, 335, 392 Annotation: Subtype: Link Rect: 340, 385, 347, 392 Annotation: Subtype: Link Rect: 327, 377, 459, 384 Annotation: Subtype: Link Rect: 463, 377, 550, 384 Annotation: Subtype: Link Rect: 554, 377, 562, 384 Annotation: Subtype: Link Rect: 327, 369, 350, 376 Annotation: Subtype: Link Rect: 354, 369, 379, 376 Annotation: Subtype: Link Rect: 382, 369, 526, 376 Annotation: Subtype: Link Rect: 530, 369, 562, 376 Annotation: Subtype: Link Rect: 327, 362, 562, 368 Annotation: Subtype: Link Rect: 327, 354, 562, 360 Annotation: Subtype: Link Rect: 327, 346, 391, 352 Annotation: Subtype: Link Rect: 396, 346, 407, 352 Annotation: Subtype: Link Rect: 327, 338, 347, 344 Annotation: Subtype: Link Rect: 351, 338, 508, 344 Annotation: Subtype: Link Rect: 512, 338, 562, 344 Annotation: Subtype: Link Rect: 327, 330, 562, 336 Annotation: Subtype: Link Rect: 327, 322, 562, 328 Annotation: Subtype: Link Rect: 327, 314, 453, 320 Annotation: Subtype: Link Rect: 458, 314, 470, 320 Annotation: Subtype: Link Rect: 327, 306, 348, 312 Annotation: Subtype: Link Rect: 352, 306, 483, 312 Annotation: Subtype: Link Rect: 487, 306, 562, 312 Annotation: Subtype: Link Rect: 327, 298, 562, 304 Annotation: Subtype: Link Rect: 327, 290, 562, 296 Annotation: Subtype: Link Rect: 327, 282, 513, 288 Annotation: Subtype: Link Rect: 518, 282, 530, 288 Annotation: Subtype: Link Rect: 327, 274, 562, 280 Annotation: Subtype: Link Rect: 327, 266, 562, 272 Annotation: Subtype: Link Rect: 327, 258, 357, 264 Annotation: Subtype: Link Rect: 362, 258, 373, 264 Annotation: Subtype: Link Rect: 327, 250, 408, 256 Annotation: Subtype: Link Rect: 412, 250, 562, 256 Annotation: Subtype: Link Rect: 327, 242, 562, 248 Annotation: Subtype: Link Rect: 327, 234, 457, 240 Annotation: Subtype: Link Rect: 462, 234, 478, 240 Annotation: Subtype: Link Rect: 327, 226, 562, 232 Annotation: Subtype: Link Rect: 327, 218, 562, 224 Annotation: Subtype: Link Rect: 327, 210, 471, 216 Annotation: Subtype: Link Rect: 476, 210, 487, 216 Annotation: Subtype: Link Rect: 327, 202, 562, 208 Annotation: Subtype: Link Rect: 327, 194, 562, 201 Annotation: Subtype: Link Rect: 327, 186, 562, 193 Annotation: Subtype: Link Rect: 327, 178, 391, 185 Annotation: Subtype: Link Rect: 396, 178, 407, 185 Annotation: Subtype: Link Rect: 327, 170, 562, 177 Annotation: Subtype: Link Rect: 327, 162, 562, 169 Annotation: Subtype: Link Rect: 327, 154, 523, 161 Annotation: Subtype: Link Rect: 528, 154, 535, 161 Annotation: Subtype: Link Rect: 327, 146, 562, 153 Annotation: Subtype: Link Rect: 327, 138, 343, 145 Annotation: Subtype: Link Rect: 346, 138, 562, 145 Annotation: Subtype: Link Rect: 327, 130, 562, 137 Annotation: Subtype: Link Rect: 327, 122, 408, 129 Annotation: Subtype: Link Rect: 413, 122, 421, 129 Annotation: Subtype: Link Rect: 327, 114, 562, 121 Annotation: Subtype: Link Rect: 327, 106, 562, 113 Annotation: Subtype: Link Rect: 327, 98, 562, 105 Annotation: Subtype: Link Rect: 327, 91, 339, 97 Annotation: Subtype: Link Rect: 344, 91, 355, 97 Annotation: Subtype: Link Rect: 327, 83, 562, 89 Annotation: Subtype: Link Rect: 327, 75, 562, 81 Annotation: Subtype: Link Rect: 327, 67, 562, 73 Annotation: Subtype: Link Rect: 327, 59, 401, 65 Annotation: Subtype: Link Rect: 406, 59, 417, 65 Annotation: Subtype: Link Rect: 327, 51, 562, 57 Page: Label: 64 Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 49, 720, 283, 727 Annotation: Subtype: Link Rect: 49, 712, 283, 719 Annotation: Subtype: Link Rect: 49, 704, 86, 711 Annotation: Subtype: Link Rect: 91, 704, 102, 711 Annotation: Subtype: Link Rect: 49, 696, 283, 703 Annotation: Subtype: Link Rect: 49, 688, 283, 695 Annotation: Subtype: Link Rect: 49, 680, 60, 687 Annotation: Subtype: Link Rect: 65, 680, 76, 687 Annotation: Subtype: Link Rect: 49, 672, 65, 679 Annotation: Subtype: Link Rect: 69, 672, 283, 679 Annotation: Subtype: Link Rect: 49, 664, 79, 671 Annotation: Subtype: Link Rect: 82, 664, 133, 671 Annotation: Subtype: Link Rect: 137, 664, 182, 671 Annotation: Subtype: Link Rect: 186, 664, 283, 671 Annotation: Subtype: Link Rect: 49, 656, 283, 663 Annotation: Subtype: Link Rect: 49, 648, 218, 655 Annotation: Subtype: Link Rect: 223, 648, 231, 655 Annotation: Subtype: Link Rect: 49, 640, 283, 647 Annotation: Subtype: Link Rect: 49, 632, 283, 639 Annotation: Subtype: Link Rect: 49, 625, 202, 631 Annotation: Subtype: Link Rect: 207, 625, 219, 631 Annotation: Subtype: Link Rect: 49, 617, 64, 623 Annotation: Subtype: Link Rect: 67, 617, 176, 623 Annotation: Subtype: Link Rect: 179, 617, 283, 623 Annotation: Subtype: Link Rect: 49, 609, 283, 615 Annotation: Subtype: Link Rect: 49, 601, 96, 607 Annotation: Subtype: Link Rect: 101, 601, 112, 607 Annotation: Subtype: Link Rect: 49, 593, 283, 599 Annotation: Subtype: Link Rect: 49, 585, 257, 591 Annotation: Subtype: Link Rect: 262, 585, 270, 591 Annotation: Subtype: Link Rect: 49, 577, 283, 583 Annotation: Subtype: Link Rect: 49, 569, 260, 575 Annotation: Subtype: Link Rect: 265, 569, 272, 575 Annotation: Subtype: Link Rect: 318, 720, 552, 727 Annotation: Subtype: Link Rect: 318, 712, 552, 719 Annotation: Subtype: Link Rect: 318, 704, 487, 711 Annotation: Subtype: Link Rect: 492, 704, 504, 711 Annotation: Subtype: Link Rect: 318, 696, 552, 703 Annotation: Subtype: Link Rect: 318, 688, 552, 695 Annotation: Subtype: Link Rect: 318, 680, 552, 687 Annotation: Subtype: Link Rect: 318, 672, 429, 679 Annotation: Subtype: Link Rect: 434, 672, 449, 679 Annotation: Subtype: Link Rect: 318, 664, 552, 671 Annotation: Subtype: Link Rect: 318, 656, 552, 663 Annotation: Subtype: Link Rect: 318, 648, 552, 655 Annotation: Subtype: Link Rect: 318, 640, 329, 647 Annotation: Subtype: Link Rect: 334, 640, 345, 647 Annotation: Subtype: Link Rect: 318, 632, 472, 639 Annotation: Subtype: Link Rect: 476, 632, 552, 639 Annotation: Subtype: Link Rect: 318, 625, 552, 631 Annotation: Subtype: Link Rect: 318, 617, 552, 623 Annotation: Subtype: Link Rect: 318, 609, 329, 615 Annotation: Subtype: Link Rect: 334, 609, 345, 615 Annotation: Subtype: Link Rect: 318, 601, 552, 607 Annotation: Subtype: Link Rect: 318, 593, 552, 599 Annotation: Subtype: Link Rect: 318, 585, 380, 591 Annotation: Subtype: Link Rect: 383, 585, 552, 591 Annotation: Subtype: Link Rect: 318, 577, 445, 583 Checksum: ded01d44 Type: CRC32 Checksum: af75f53c29e70f3d954f97514a778243 Type: MD5 Checksum: 56e46ae84481963f87fc2eb2292f129356d52d16 Type: SHA-1