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Item: Title: Cells. Item: Title: EV isolation by size exclusion chromatography (SEC). Item: Title: EV enrichment by Ultracentrifugation. Item: Title: Flow cytometry assays. Item: Title: Protein concentration measurement. Item: Title: Nanoparticle tracking analysis. Item: Title: Sample processing for Transmission Electron Microscopy (TEM). Item: Title: Statistical analysis. Item: Title: Results Children: Item: Title: Bead-assisted flow cytometry can be used as a highly sensitive semi-quantitative method for EV analysis. Item: Title: Comparison of bead-assisted flow cytometry with Nanoparticle Tracking Analysis. Item: Title: Bead-assisted flow cytometry can be employed in biofluid-derived samples. Item: Title: The use of bead-assisted flow cytometry in protein-containing samples. Item: Title: Discussion Item: Title: Acknowledgements Item: Title: Figure 1 SEC-elution profile of EV samples. Item: Title: Figure 2 Characterization of EVs. Item: Title: Figure 3 Analysis of antibody-staining detection of EV surface molecules in flow cytometry assays. Item: Title: Figure 4 MFI values linearly correlate with EV content. Item: Title: Figure 5 Comparison of flow cytometry-based and NTA quantitative analyses. Item: Title: Figure 6 MFI values obtained in human urine samples also correlate with dilution factor and protein concentration. Item: Title: Figure 7 MFI determination is impaired by high levels of protein contamination. 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doi:10.1038/s41598-017-11249-2 A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles Henar Suamp#x000E1;rez Ana Gamp#x000E1;mez-Valero Raquel Reyes Soraya Lamp#x000F3;pez-Martamp#x000ED;n Maramp#x000ED;a Josefa Rodramp#x000ED;guez Josamp#x000E9; L. 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