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Title:          Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-creating or spacefilling mutations
Subject:        Scientific Reports, doi:10.1038/s41598-017-04345-w
Keywords:       
Author:         Pablo Guerra
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     Item: 
      Title: Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-creating or spacefilling mutat ...
      Children: 
       Item: 
        Title: Results and Discussion
        Children: 
         Item: 
          Title: Compared local and global mechanical effects of cavity-creating N170A and spacefilling L172W mutations in the MVM capsid. 
         Item: 
          Title: Compared effects on capsid conformational dynamics of N170A and L172W mutations. 
         Item: 
          Title: Crystal structure of a mechanically stiffened N170A mutant MVM capsid, and comparison with the non-mutated capsid structure ...
         Item: 
          Title: Compared structural effects of N170A and L172W mutations in the MVM capsid. 
         Item: 
          Title: Structural effects that may underlie mutation-induced mechanical stiffening of a virus particle. 
         Item: 
          Title: Biological implications. 
         Item: 
          Title: Nanotechnological and biomedical implications. 
       Item: 
        Title: Conclusion
       Item: 
        Title: Methods
        Children: 
         Item: 
          Title: Recombinant plasmids and mutagenesis. 
         Item: 
          Title: Expression and purification of MVM capsids. 
         Item: 
          Title: Crystallization, data collection and processing. 
         Item: 
          Title: Structure solution and refinement. 
         Item: 
          Title: Atomic force microscopy. 
         Item: 
          Title: Statistical analysis of mechanical data. 
       Item: 
        Title: Acknowledgements
       Item: 
        Title: Figure 1 The MVM capsid.
       Item: 
        Title: Figure 2 Topography and mechanical stiffness of wt and mutant MVM capsids.
       Item: 
        Title: Figure 3 2Fo–Fc averaged electron density corresponding to the N170A mutant MVMp capsid structure (1.
       Item: 
        Title: Figure 4 Surface representation of the region around a S5 axis in the MVMp capsid.
       Item: 
        Title: Figure 5 Comparison between the VP2 structure in the wt and mutant N170A MVMp capsids.
       Item: 
        Title: Figure 6 Structure of VP2 in the MVM capsid, color-coded according to normalized B-factors per residue.
       Item: 
        Title: Table 1 Elastic constant ks valuesa determined for MVM capsids (wt and N170A and L172W mutants) along S2, S3 or S5 axes.
       Item: 
        Title: Table 2 X-ray data collection and refinement statistics for the N170A mutant MVMp capsid.
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