12fffd3b5ec1a98aec03786d6159f3db pmc_29104531.pdf 5d0008c473b79202bf6cfae173e16169000789be pmc_29104531.pdf 715765e483322f71b6170c077418f20f4b5f9045c6b2d08a8470247fc1466f4c pmc_29104531.pdf Title: The Repression of Atoh1 by Neurogenin1 during Inner Ear Development Subject: Atonal homolog 1 (Atoh1) and Neurogenin1 (Neurog1) are basic Helix-Loop-Helix (bHLH) transcription factors crucial for the generation of hair cells (HCs) and neurons in the inner ear. Keywords: neurogenesis, neural progenitors, cell fate, bHLH factors, Neurog1, sensory regeneration Author: Fernando Giraldez and Gina Abelló Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.12 CreationDate: Fri Oct 20 12:09:50 2017 CEST ModDate: Fri Oct 20 12:13:55 2017 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 2867277 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KSFLII+MinionPro-Regular Type 1 Custom yes yes no 739 0 MNTPWH+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 742 0 CITHRQ+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 745 0 OQAWSQ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 748 0 WFACWU+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 751 0 MKVOIJ+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 754 0 VBUIAF+MinionPro-Bold 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during Inner Ear Development Children: Item: Title: INTRODUCTION Item: Title: MATERIALS AND METHODS Children: Item: Title: In Ovo Electroporation and Plasmids Item: Title: β-galactosidase and Luciferase Reporter Assays Item: Title: Site Directed Mutagenesis Item: Title: Neurog1 Deletions and E-box Reporter Systems Item: Title: P19 Transfection and Luciferase Reporter Assay Item: Title: ATAC-Seq Analysis Item: Title: Immunoprecipitation and Mass Spectrometry Item: Title: β-galactosidase Staining and Immuno -histochemistry Item: Title: Immunoblot Analysis Item: Title: In Vitro Cultures of Otic Vesicles Item: Title: Statistics Item: Title: RESULTS Children: Item: Title: Neurog1 Repression of Atoh1 Autoregulatory Loop Occurs at the 3'Atoh1 Enhancer Item: Title: Dissecting the 3' Atoh1 Enhancer: Enhancer B at the Core of Repression Item: Title: Repression by Neurog1 Requires the CAN Region Item: Title: Neurog1 Does Not Require Direct Binding to DNA for Atoh1 Repression Item: Title: Neurog1 Requires the Helix-1 Dimerization Domain for Atoh1 Repression Item: Title: Neurog1 Does Not Interact with Atoh1 Protein Item: Title: Neurog1 Reduces Atoh1 Protein Levels Item: Title: DISCUSSION Children: Item: Title: Neurons vs. Hair Cells Item: Title: The Enhancer B Accounts for the Atoh1 Autoregulatory Loop Item: Title: The Flanking Region of E-box A Selects the Binding Affinity of Atoh1 Item: Title: The Mechanism of Atoh1 Repression by Neurog1 Item: Title: AUTHOR CONTRIBUTIONS Item: Title: FUNDING Item: Title: ACKNOWLEDGMENTS Item: Title: SUPPLEMENTARY MATERIAL Item: Title: REFERENCES Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: HBPTJU+MinionPro-It FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: HBPTJU+MinionPro-It Flags: Symbolic FontBBox: -199, -358, 1684, 1002 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MKVOIJ+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 225 FontDescriptor: FontName: 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WFACWU+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -164, -216, 1078, 975 FontFile: true EncodingDictionary: Differences: true XMP: application/pdf Fernando Giraldez and Gina Abelló Atonal homolog 1 (Atoh1) and Neurogenin1 (Neurog1) are basic Helix-Loop-Helix (bHLH) transcription factors crucial for the generation of hair cells (HCs) and neurons in the inner ear. 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