dc03cefb0a6f28a4862cfb7a4e036ced pmc_28767100.pdf 89d50d69be203885c68ee9726e8ca6d4fc42fae5 pmc_28767100.pdf Title: Adsorption of Milk Proteins (-Casein and -Lactoglobulin) and BSA onto Hydrophobic Surfaces Subject: Here, we study films of proteins over planar surfaces and protein-coated microspheres obtained from the adsorption of three different proteins (-casein, -lactoglobulin and bovine serum albumin (BSA)). The investigation of protein films in planar surfaces is performed by combining quartz crystal microbalance (QCM) and atomic force microscopy (AFM) measurements with all-atomic molecular dynamics (MD) simulations. We found that BSA and -lactoglobulin form compact monolayers, almost without interstices between the proteins. However, -casein adsorbs forming multilayers. The study of the electrokinetic mobility of protein-coated latex microspheres shows substantial condensation of ions from the buffer over the complexes, as predicted from ion condensation theories. The electrokinetic behavior of the latex-protein complexes is dominated by the charge of the proteins and the phenomenon of ion condensation, whereas the charge of the latex colloids plays only a minor role. Keywords: proteins; QCM; MD simulations; hydrophobic effect; ion condensation; electrokinetic mobility Author: Firstname Lastname, Firstname Lastname and Firstname Lastname Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed Aug 2 14:29:25 2017 CEST ModDate: Wed Aug 2 14:29:25 2017 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 25 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 9559921 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KNWKSS+URWPalladioL-Ital Type 1 Custom yes yes no 109 0 ZYFLNN+URWPalladioL-Bold Type 1 Custom yes yes no 110 0 IUOUHH+PazoMath-BoldItalic Type 1 Builtin yes yes no 111 0 UXEXHX+URWPalladioL-Roma Type 1 Custom yes yes no 112 0 WYOCPL+PazoMath-Italic Type 1 Builtin yes yes no 113 0 OAZCXF+CMSY10 Type 1 Builtin yes yes no 172 0 JVWKXI+PazoMath Type 1 Builtin yes yes no 173 0 ETYAWE+f-0-0 Type 1C WinAnsi yes yes no 181 0 AEOBWO+f-0-0 Type 1C WinAnsi yes yes no 219 0 GYMYXO+DejaVuSans-Bold TrueType WinAnsi yes yes yes 228 0 WPDJMJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 250 0 PDXFNG+PazoMath-Bold Type 1 Builtin yes yes no 251 0 SOSTRQ+CMR10 Type 1 Builtin yes yes no 275 0 [none] Type 3 Custom yes no no 281 0 MTMIIH+f-0-0 Type 1C WinAnsi yes yes no 282 0 TGGLQE+f-0-1 Type 1C Custom yes yes yes 283 0 OFTRMJ+f-1-1 Type 1C Custom yes yes no 284 0 UPGGKQ+DejaVuSans TrueType WinAnsi yes yes yes 285 0 GPAHBZ+DejaVuSans-Bold TrueType WinAnsi yes yes yes 286 0 KGMBUU+DejaVuSans-Bold TrueType WinAnsi yes yes yes 364 0 KWACEW+DejaVuSans TrueType WinAnsi yes yes yes 391 0 ZTDNWT+DejaVuSans-Bold TrueType WinAnsi yes yes yes 392 0 XASGME+DejaVuSans-Bold TrueType WinAnsi yes yes yes 393 0 GZTIEH+f-2-0 Type 1C WinAnsi yes yes no 394 0 WRZRNG+f-2-1 Type 1C Custom yes yes yes 395 0 [none] Type 3 Custom yes no no 438 0 IWFFLP+DejaVuSans-Bold TrueType WinAnsi yes yes yes 439 0 WIISTP+f-1-0 Type 1C WinAnsi yes yes no 440 0 ZAJCPJ+f-2-0 Type 1C WinAnsi yes yes no 441 0 WRZRNG+f-2-1 Type 1C Custom yes yes yes 442 0 ITTABK+f-3-0 Type 1C WinAnsi yes yes no 443 0 HFCUQU+f-0-0 Type 1C WinAnsi yes yes no 477 0 SFIMNB+DejaVuSans TrueType WinAnsi yes yes yes 478 0 EBYDXZ+DejaVuSans TrueType WinAnsi yes yes yes 479 0 NEMJLD+DejaVuSans-Oblique TrueType WinAnsi yes yes yes 480 0 AIAXUC+DejaVuSans-Bold TrueType WinAnsi yes yes yes 481 0 XMETLD+f-4-0 Type 1C WinAnsi yes yes no 482 0 UCQFDI+f-4-1 Type 1C Custom yes yes yes 483 0 PWVRMV+f-5-1 Type 1C Custom yes yes no 484 0 DURHUM+CMMI10 Type 1 Builtin yes yes no 535 0 FHQYHH+f-0-0 Type 1C WinAnsi yes yes no 560 0 TGGLQE+f-0-1 Type 1C Custom yes yes yes 561 0 EZRQTK+f-1-1 Type 1C Custom yes yes no 562 0 FELFRC+f-2-0 Type 1C WinAnsi yes yes no 563 0 ZBMMMF+DejaVuSans-Bold TrueType WinAnsi yes yes yes 564 0 RQTYCY+DejaVuSans TrueType WinAnsi yes yes yes 565 0 CZAHBN+DejaVuSans TrueType WinAnsi yes yes yes 566 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 606 0 LVTKIT+CMEX10 Type 1 Builtin yes yes no 627 0 WEIJUK+CMSY9 Type 1 Builtin yes yes no 743 0 java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary at edu.harvard.hul.ois.jhove.module.PdfModule.readDocCatalogDict(PdfModule.java:1284) at edu.harvard.hul.ois.jhove.module.PdfModule.parse(PdfModule.java:525) at edu.harvard.hul.ois.jhove.JhoveBase.processFile(JhoveBase.java:825) at edu.harvard.hul.ois.jhove.JhoveBase.process(JhoveBase.java:614) at edu.harvard.hul.ois.jhove.JhoveBase.dispatch(JhoveBase.java:465) at Jhove.main(Jhove.java:296) Jhove (Rel. 1.6, 2011-01-04) Date: 2019-02-14 17:57:00 CET RepresentationInformation: pmc_28767100.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2018-02-08 07:53:28 CET Size: 9559921 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: f7b2b88f Type: CRC32 Checksum: dc03cefb0a6f28a4862cfb7a4e036ced Type: MD5 Checksum: 89d50d69be203885c68ee9726e8ca6d4fc42fae5 Type: SHA-1