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Title:          Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses
Subject:        Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation.
Keywords:       tolerance, monitoring, proliferation, antigen-specific, T cells, transplantation, autoimmune diseases, immune-therapies
Author:         Anja Ten Brinke
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     Item: 
      Title: Monitoring T-Cell Responses in Translational Studies: Optimization 
of Dye-Based Proliferation Assay for Evaluation of Antigenⴀ匀瀀攀挀椀昀椀挀 刀攀猀瀀漀渀猀攀猩㸾㰼⽄⡦業浵ⴰ㠭〱㠷〮楮摤㨮㐳㠴〺㤱㠩⽓⽇潔漾㸼㰯䄠㜱㜠〠刯乥硴‶㜲‰⁒⽐慲敮琠㘴㠠〠刯呩瑬攨䥮瑲潤畣瑩潮
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        Title: Introduction
       Item: 
        Title: Materials and Equipment
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         Item: 
          Title: Subjects
         Item: 
          Title: Cell Preparations
         Item: 
          Title: Dye-Labeling and Proliferation
          Children: 
           Item: 
            Title: Polyclonal Stimulation
           Item: 
            Title: Staphylococcal Enterotoxin B (SEB) Stimulation
           Item: 
            Title: AlloAg and Pathogen-Specific Ag Stimulation
           Item: 
            Title: AutoAg Stimulation
         Item: 
          Title: Ovalbumin (OVA) Stimulation for Frequency Calculation
          Children: 
           Item: 
            Title: Responder Cells
           Item: 
            Title: Stimulator Cells
           Item: 
            Title: CFSE Labeling for Frequency Calculation
           Item: 
            Title: ELISpot Assay for Frequency Calculation
           Item: 
            Title: Fascia
           Item: 
            Title: IFN-γ Production
         Item: 
          Title: Statistics
       Item: 
        Title: Stepwise Procedures
        Children: 
         Item: 
          Title: Proliferation Dye Selection: Defining Optimal Dye and Labeling Concentration
         Item: 
          Title: Peaks Resolution: Autofluorescence versus Cell Proliferation
         Item: 
          Title: Assay Setup
          Children: 
           Item: 
            Title: Definition of the Number of Cells/Wells to Seed
           Item: 
            Title: Proper Selection of the Responder, Stimulator Cells and Optimal Time Point to Monitor Proliferation
         Item: 
          Title: Acquisition Setup
         Item: 
          Title: Optimal Parameters for the Analysis of Proliferation Dye Data
       Item: 
        Title: Anticipated Results (Pitfalls, Artifacts, and Troubleshooting)
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         Item: 
          Title: Sensitivity of the Dye Proliferation Assay Compared to Other Approaches
       Item: 
        Title: Concluding Remarks
       Item: 
        Title: Ethics Statement
       Item: 
        Title: Author Contributions
       Item: 
        Title: Acknowledgments
       Item: 
        Title: Funding
       Item: 
        Title: Supplementary Material
       Item: 
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  Checksum: d3e277a9
   Type: CRC32
  Checksum: 84aad11a7a754e144968b2841a5c18c5
   Type: MD5
  Checksum: 30df2ff07fb14db70d059d5a2c866ec2b924cfec
   Type: SHA-1
  Checksum: 5ab13ce5a8e14bfd3123c267b8783e59c72b52ba70724a869c25a267daf3def4
   Type: SHA-256