8162edcf514764643663ee7b23326a3e sustainability_a2018v10n7a2433.pdf 0e60e272155df2278025d8eae84df2be22f7da11 sustainability_a2018v10n7a2433.pdf 77a29ef7ca73a8d0bdb2d8aa0f5077d16449e3d995d19b85c33e4ff55bd3ed40 sustainability_a2018v10n7a2433.pdf Title: Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste Subject: Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation. Keywords: cellulase; digestate; solid-state fermentation; specialized inoculum; xylanase Author: Laura Mejias, Alejandra Cerda, Raquel Barrena, Teresa Gea and Antoni Sánchez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Thu Jul 12 08:20:59 2018 CEST ModDate: Mon Oct 22 14:32:50 2018 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1992717 bytes Optimized: yes PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 541 0 GEPBNV+URWPalladioL-Ital Type 1 Custom yes yes no 545 0 JIPILX+URWPalladioL-Bold Type 1 Custom yes yes no 548 0 JASHAJ+URWPalladioL-Roma Type 1 Custom yes yes no 551 0 OXBKYU+TeXGyreAdventor-Bold Type 1 Custom yes yes no 555 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 558 0 IZBVAO+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 562 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 569 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 572 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 575 0 OJYQXC+CMSY10 Type 1 Builtin yes yes no 314 0 ABCDEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 107 0 ABCDEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 110 0 ABCDEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 113 0 CZKGAS+CMR10 Type 1 Builtin yes yes no 318 0 UYVLXV+CMEX10 Type 1 Builtin yes yes no 317 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 319 0 ABCDEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 172 0 ABCDEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 175 0 ABCDEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 178 0 ABCDEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 209 0 ABCDEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 212 0 ABCDEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 215 0 ABCDEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 228 0 ABCDEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 231 0 ABCDEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 234 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-04-12 03:45:00 CEST RepresentationInformation: sustainability_a2018v10n7a2433.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2018-10-22 14:36:42 CEST Size: 1992717 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Substrate Characterization Item: Title: Inoculum Preparation Item: Title: Solid-State Fermentation (SSF) Children: Item: Title: Experimental Set-Up Item: Title: Monitoring Parameters Item: Title: Experimental Methodology Item: Title: Analytical Methods Children: Item: Title: Enzyme Extraction Item: Title: Enzymatic Activity Determination Item: Title: Routine Methods Item: Title: Statistics Item: Title: Results and Discussion Children: Item: Title: Inoculation Requirements Determination Item: Title: SBR as Strategy to Obtain a Specialized Inoculum for Cellulase and Xylanase Production Item: Title: Conclusions Item: Title: References Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: GEPBNV+URWPalladioL-Ital FontSubset: true FirstChar: 40 LastChar: 150 FontDescriptor: FontName: GEPBNV+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JIPILX+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: JIPILX+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JASHAJ+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 241 FontDescriptor: FontName: JASHAJ+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OJYQXC+CMSY10 FontSubset: true FirstChar: 0 LastChar: 14 FontDescriptor: FontName: OJYQXC+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: OXBKYU+TeXGyreAdventor-Bold FontSubset: true FirstChar: 68 LastChar: 73 FontDescriptor: FontName: OXBKYU+TeXGyreAdventor-Bold Flags: Symbolic FontBBox: -679, -304, 1661, 1236 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MWHIBA+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: MWHIBA+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: UYVLXV+CMEX10 FontSubset: true FirstChar: 90 LastChar: 90 FontDescriptor: FontName: UYVLXV+CMEX10 Flags: Symbolic FontBBox: -24, -2960, 1454, 772 FontFile: true Font: BaseFont: AIQCCL+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 161 FontDescriptor: FontName: AIQCCL+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: CZKGAS+CMR10 FontSubset: true FirstChar: 40 LastChar: 61 FontDescriptor: FontName: CZKGAS+CMR10 Flags: Symbolic FontBBox: -40, -250, 1009, 750 FontFile: true Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true XMP: application/pdf Laura Mejias, Alejandra Cerda, Raquel Barrena, Teresa Gea and Antoni Sánchez Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation. Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste 2018-07-12T14:20:59+08:00 LaTeX with hyperref package 2018-10-22T14:32:50+02:00 2018-10-22T14:32:50+02:00 cellulase; digestate; solid-state fermentation; specialized inoculum; xylanase pdfTeX-1.40.15 False This is pdfTeX, Version 3.14159265-2.6-1.40.15 (TeX Live 2014/W32TeX) kpathsea version 6.2.0 uuid:6c29dca9-b8e5-4937-bf7b-94a22afd56fe uuid:52c6fa82-3156-4ef5-9918-e6350418b23b Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 218, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 385, 639, 398, 654 Annotation: Subtype: Link Rect: 467, 524, 519, 545 Annotation: Subtype: Link Rect: 508, 260, 515, 269 ActionDest: 65 Annotation: Subtype: Link Rect: 366, 192, 373, 201 ActionDest: 65 Annotation: Subtype: Link Rect: 263, 165, 270, 174 ActionDest: 65 Annotation: Subtype: Link Rect: 222, 138, 229, 147 ActionDest: 65 Annotation: Subtype: Link Rect: 229, 138, 236, 147 ActionDest: 65 Annotation: Subtype: Link Rect: 422, 136, 517, 148 Annotation: Subtype: Link Rect: 296, 84, 303, 93 ActionDest: 65 Annotation: Subtype: Link Rect: 187, 38, 260, 50 Annotation: Subtype: Link Rect: 378, 38, 519, 50 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 125, 729, 132, 738 ActionDest: 65 Annotation: Subtype: Link Rect: 132, 729, 139, 738 ActionDest: 65 Annotation: Subtype: Link Rect: 390, 715, 397, 724 ActionDest: 65 Annotation: Subtype: Link Rect: 397, 715, 409, 724 ActionDest: 65 Annotation: Subtype: Link Rect: 432, 675, 444, 684 ActionDest: 65 Annotation: Subtype: Link Rect: 418, 648, 430, 657 ActionDest: 65 Annotation: Subtype: Link Rect: 213, 634, 225, 643 ActionDest: 65 Annotation: Subtype: Link Rect: 188, 621, 200, 630 ActionDest: 65 Annotation: Subtype: Link Rect: 203, 621, 215, 630 ActionDest: 65 Annotation: Subtype: Link Rect: 503, 607, 515, 616 ActionDest: 65 Annotation: Subtype: Link Rect: 450, 594, 462, 603 ActionDest: 65 Annotation: Subtype: Link Rect: 165, 298, 177, 307 ActionDest: 65 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 140, 657, 147, 669 ActionDest: 4 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 332, 710, 344, 718 ActionDest: 65 Annotation: Subtype: Link Rect: 401, 571, 412, 580 ActionDest: 65 Annotation: Subtype: Link Rect: 503, 493, 515, 502 ActionDest: 68 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 134, 729, 146, 738 ActionDest: 68 Annotation: Subtype: Link Rect: 106, 594, 118, 603 ActionDest: 65 Annotation: Subtype: Link Rect: 119, 594, 130, 603 ActionDest: 68 Annotation: Subtype: Link Rect: 111, 447, 123, 456 ActionDest: 68 Annotation: Subtype: Link Rect: 116, 393, 128, 402 ActionDest: 65 Annotation: Subtype: Link Rect: 129, 337, 141, 346 ActionDest: 68 Annotation: Subtype: Link Rect: 264, 283, 276, 292 ActionDest: 68 Annotation: Subtype: Link Rect: 503, 228, 515, 238 ActionDest: 68 Annotation: Subtype: Link Rect: 128, 118, 140, 127 ActionDest: 68 Annotation: Subtype: Link Rect: 158, 105, 170, 114 ActionDest: 68 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 390, 614, 397, 626 ActionDest: 28 Annotation: Subtype: Link Rect: 443, 614, 450, 626 ActionDest: 28 Annotation: Subtype: Link Rect: 136, 562, 148, 571 ActionDest: 68 Annotation: Subtype: Link Rect: 299, 546, 306, 560 ActionDest: 28 Annotation: Subtype: Link Rect: 276, 522, 288, 531 ActionDest: 65 Annotation: Subtype: Link Rect: 298, 506, 305, 518 ActionDest: 25 Annotation: Subtype: Link Rect: 469, 454, 481, 463 ActionDest: 68 Annotation: Subtype: Link Rect: 421, 438, 428, 450 ActionDest: 28 Annotation: Subtype: Link Rect: 240, 346, 252, 355 ActionDest: 65 Annotation: Subtype: Link Rect: 129, 330, 136, 342 ActionDest: 28 Annotation: Subtype: Link Rect: 503, 319, 515, 328 ActionDest: 65 Annotation: Subtype: Link Rect: 323, 306, 335, 315 ActionDest: 68 Annotation: Subtype: Link Rect: 413, 263, 420, 275 ActionDest: 28 Annotation: Subtype: Link Rect: 330, 209, 337, 221 ActionDest: 28 Annotation: Subtype: Link Rect: 471, 171, 482, 180 ActionDest: 68 Annotation: Subtype: Link Rect: 235, 130, 247, 139 ActionDest: 68 Annotation: Subtype: Link Rect: 157, 100, 164, 112 ActionDest: 25 Annotation: Subtype: Link Rect: 153, 90, 165, 99 ActionDest: 68 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 96, 455, 107, 463 ActionDest: 65 Annotation: Subtype: Link Rect: 96, 428, 107, 435 ActionDest: 68 Annotation: Subtype: Link Rect: 96, 391, 107, 398 ActionDest: 68 Annotation: Subtype: Link Rect: 96, 359, 107, 366 ActionDest: 65 Annotation: Subtype: Link Rect: 96, 336, 107, 343 ActionDest: 68 Annotation: Subtype: Link Rect: 96, 309, 107, 316 ActionDest: 68 Annotation: Subtype: Link Rect: 96, 276, 107, 284 ActionDest: 68 Annotation: Subtype: Link Rect: 96, 244, 107, 252 ActionDest: 65 Annotation: Subtype: Link Rect: 96, 208, 107, 215 ActionDest: 65 Annotation: Subtype: Link Rect: 96, 166, 107, 173 ActionDest: 65 Page: Sequence: 8 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 490, 648, 502, 657 ActionDest: 68 Annotation: Subtype: Link Rect: 503, 648, 515, 657 ActionDest: 68 Annotation: Subtype: Link Rect: 466, 526, 478, 535 ActionDest: 65 Annotation: Subtype: Link Rect: 478, 526, 490, 535 ActionDest: 65 Annotation: Subtype: Link Rect: 491, 526, 503, 535 ActionDest: 65 Annotation: Subtype: Link Rect: 503, 526, 515, 535 ActionDest: 68 Annotation: Subtype: Link Rect: 421, 497, 428, 509 ActionDest: 25 Annotation: Subtype: Link Rect: 143, 486, 155, 495 ActionDest: 71 Annotation: Subtype: Link Rect: 367, 375, 379, 385 ActionDest: 65 Annotation: Subtype: Link Rect: 266, 321, 273, 330 ActionDest: 65 Annotation: Subtype: Link Rect: 132, 265, 140, 277 ActionDest: 42 Annotation: Subtype: Link Rect: 160, 265, 167, 277 ActionDest: 52 Annotation: Subtype: Link Rect: 299, 227, 311, 236 ActionDest: 65 Annotation: Subtype: Link Rect: 311, 227, 323, 236 ActionDest: 71 Annotation: Subtype: Link Rect: 304, 173, 316, 182 ActionDest: 71 Annotation: Subtype: Link Rect: 140, 76, 147, 88 ActionDest: 25 Annotation: Subtype: Link Rect: 294, 78, 305, 87 ActionDest: 71 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 390, 702, 402, 711 ActionDest: 68 Annotation: Subtype: Link Rect: 403, 702, 415, 711 ActionDest: 71 Annotation: Subtype: Link Rect: 281, 675, 293, 684 ActionDest: 65 Annotation: Subtype: Link Rect: 503, 621, 515, 630 ActionDest: 71 Annotation: Subtype: Link Rect: 144, 607, 156, 616 ActionDest: 71 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 423, 740, 430, 752 ActionDest: 52 Annotation: Subtype: Link Rect: 503, 742, 515, 751 ActionDest: 65 Annotation: Subtype: Link Rect: 503, 713, 510, 725 ActionDest: 28 Annotation: Subtype: Link Rect: 138, 672, 145, 684 ActionDest: 52 Annotation: Subtype: Link Rect: 238, 634, 250, 643 ActionDest: 65 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 134, 648, 146, 657 ActionDest: 65 Annotation: Subtype: Link Rect: 146, 648, 158, 657 ActionDest: 65 Annotation: Subtype: Link Rect: 159, 648, 171, 657 ActionDest: 71 Annotation: Subtype: Link Rect: 102, 567, 114, 576 ActionDest: 71 Annotation: Subtype: Link Rect: 107, 510, 114, 522 ActionDest: 52 Annotation: Subtype: Link Rect: 194, 486, 206, 495 ActionDest: 65 Annotation: Subtype: Link Rect: 206, 486, 218, 495 ActionDest: 71 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 432, 721, 519, 732 Annotation: Subtype: Link Rect: 97, 708, 263, 719 Annotation: Subtype: Link Rect: 307, 669, 344, 680 Annotation: Subtype: Link Rect: 350, 669, 387, 680 Annotation: Subtype: Link Rect: 358, 631, 395, 642 Annotation: Subtype: Link Rect: 402, 631, 438, 642 Annotation: Subtype: Link Rect: 429, 579, 466, 590 Annotation: Subtype: Link Rect: 267, 540, 304, 551 Annotation: Subtype: Link Rect: 281, 515, 319, 525 Annotation: Subtype: Link Rect: 325, 515, 361, 525 Annotation: Subtype: Link Rect: 325, 489, 362, 500 Annotation: Subtype: Link Rect: 409, 476, 446, 487 Annotation: Subtype: Link Rect: 453, 476, 489, 487 Annotation: Subtype: Link Rect: 275, 437, 313, 448 Annotation: Subtype: Link Rect: 413, 411, 451, 422 Annotation: Subtype: Link Rect: 406, 385, 444, 396 Annotation: Subtype: Link Rect: 238, 347, 276, 358 Annotation: Subtype: Link Rect: 282, 347, 318, 358 Annotation: Subtype: Link Rect: 100, 309, 137, 319 Annotation: Subtype: Link Rect: 136, 270, 173, 280 Annotation: Subtype: Link Rect: 275, 243, 312, 254 Annotation: Subtype: Link Rect: 479, 217, 516, 229 Annotation: Subtype: Link Rect: 244, 179, 282, 190 Annotation: Subtype: Link Rect: 227, 88, 264, 99 Annotation: Subtype: Link Rect: 270, 88, 306, 99 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 479, 727, 516, 738 Annotation: Subtype: Link Rect: 100, 715, 136, 725 Annotation: Subtype: Link Rect: 292, 676, 330, 686 Annotation: Subtype: Link Rect: 336, 676, 372, 686 Annotation: Subtype: Link Rect: 100, 638, 137, 648 Annotation: Subtype: Link Rect: 143, 638, 179, 648 Annotation: Subtype: Link Rect: 480, 611, 516, 622 Annotation: Subtype: Link Rect: 395, 598, 433, 609 Annotation: Subtype: Link Rect: 181, 560, 218, 570 Annotation: Subtype: Link Rect: 330, 533, 519, 544 Annotation: Subtype: Link Rect: 97, 521, 148, 532 Annotation: Subtype: Link Rect: 426, 495, 464, 506 Annotation: Subtype: Link Rect: 135, 457, 173, 467 Annotation: Subtype: Link Rect: 219, 430, 256, 441 Annotation: Subtype: Link Rect: 263, 430, 299, 441 Annotation: Subtype: Link Rect: 398, 404, 435, 415 Annotation: Subtype: Link Rect: 253, 366, 291, 377 Annotation: Subtype: Link Rect: 297, 366, 333, 377 Annotation: Subtype: Link Rect: 356, 315, 394, 325 Annotation: Subtype: Link Rect: 400, 315, 436, 325 Annotation: Subtype: Link Rect: 252, 276, 290, 286 Annotation: Subtype: Link Rect: 296, 276, 332, 286 Annotation: Subtype: Link Rect: 305, 237, 342, 247 Annotation: Subtype: Link Rect: 235, 198, 272, 209 Annotation: Subtype: Link Rect: 317, 159, 354, 170 Annotation: Subtype: Link Rect: 360, 159, 397, 170 Annotation: Subtype: Link Rect: 472, 133, 509, 144 Annotation: Subtype: Link Rect: 398, 94, 436, 105 Annotation: Subtype: Link Rect: 442, 94, 478, 105 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 351, 715, 388, 725 Annotation: Subtype: Link Rect: 395, 715, 431, 725 Annotation: Subtype: Link Rect: 127, 676, 164, 686 Annotation: Subtype: Link Rect: 100, 638, 137, 648 Annotation: Subtype: Link Rect: 143, 638, 179, 648 Annotation: Subtype: Link Rect: 331, 612, 368, 622 Annotation: Subtype: Link Rect: 308, 572, 345, 583 Annotation: Subtype: Link Rect: 158, 534, 196, 544 Annotation: Subtype: Link Rect: 262, 495, 300, 506 Annotation: Subtype: Link Rect: 306, 495, 342, 506 Annotation: Subtype: Link Rect: 76, 453, 166, 484 Annotation: Subtype: Link Rect: 234, 447, 424, 458 Checksum: a8abfd98 Type: CRC32 Checksum: 8162edcf514764643663ee7b23326a3e Type: MD5 Checksum: 0e60e272155df2278025d8eae84df2be22f7da11 Type: SHA-1