d55b9127826a508f5a4505da5b29596c intjoumolsci_a2018v19n5p1384.pdf f31aa29eecd80f76385eb6561fc0986cd6884bd7 intjoumolsci_a2018v19n5p1384.pdf 485300f087c87fc391d68179d71402768c0bdfaf55c612369e6e929da59f75ff intjoumolsci_a2018v19n5p1384.pdf Title: The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region Subject: Response mechanisms to external stress rely on networks of proteins able to activate specific signaling pathways to ensure the maintenance of cell proteostasis. Many of the proteins mediating this kind of response contain intrinsically disordered regions, which lack a defined structure, but still are able to interact with a wide range of clients that modulate the protein function. Some of these interactions are mediated by specific short sequences embedded in the longer disordered regions. Because the physicochemical properties that promote functional and abnormal interactions are similar, it has been shown that, in globular proteins, aggregation-prone and binding regions tend to overlap. It could be that the same principle applies for disordered protein regions. In this context, we show here that a predicted low-complexity interacting region in the disordered C-terminus of the stress response master regulator heat shock factor 1 (Hsf1) protein corresponds to a cryptic amyloid region able to self-assemble into fibrillary structures resembling those found in neurodegenerative disorders. Keywords: amyloid; Hsf1; protein aggregation; intrinsically disordered regions; Q/N-rich regions; low complexity; molecular recognition features Author: Jordi Pujols, Jaime Santos, Irantzu Pallarès and Salvador Ventura Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Mon May 7 10:11:27 2018 CEST ModDate: Fri Oct 19 11:21:35 2018 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2724784 bytes Optimized: yes PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DFFCNG+VnURWPalladioL Type 1 Custom yes yes no 646 0 HCLQQC+URWPalladioL-Ital Type 1 Custom yes yes no 650 0 QUGULU+URWPalladioL-Bold Type 1 Custom yes yes no 653 0 IGGCGS+URWPalladioL-Roma Type 1 Custom yes yes no 656 0 OXBKYU+TeXGyreAdventor-Bold Type 1 Custom yes yes no 660 0 WYEKQU+VnURWPalladioL-Bold Type 1 Custom yes yes no 663 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 667 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 669 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 678 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 681 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 684 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 132 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 135 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 138 0 Times New Roman TrueType WinAnsi no no no 141 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 147 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 415 0 VORBJF+CMSY10 Type 1 Builtin yes yes no 412 0 CRJHCU+PazoMath-Italic Type 1 Builtin yes yes no 411 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 161 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 164 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 167 0 Times New Roman TrueType WinAnsi no no no 170 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 176 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 182 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 194 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 197 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 200 0 Times New Roman TrueType WinAnsi no no no 203 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 209 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 217 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 220 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 223 0 Times New Roman TrueType WinAnsi no no no 226 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 232 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 242 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 245 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 248 0 Times New Roman TrueType WinAnsi no no no 251 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 257 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 266 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 269 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 272 0 Times New Roman TrueType WinAnsi no no no 275 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 281 0 Jhove (Rel. 1.6, 2011-01-04) Date: 2018-10-31 04:44:19 CET RepresentationInformation: intjoumolsci_a2018v19n5p1384.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2018-10-19 11:22:01 CEST Size: 2724784 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: 016ceb87 Type: CRC32 Checksum: d55b9127826a508f5a4505da5b29596c Type: MD5 Checksum: f31aa29eecd80f76385eb6561fc0986cd6884bd7 Type: SHA-1