4e57800de46c444d0b89af2a172128ee fromic_a2018m10v9p2370.pdf 77c03601ed56f752d26dddc4404fab6520b22a43 fromic_a2018m10v9p2370.pdf c697c0e4a89a8ce8f6da0d16155664759dca9a1c5bed3727d88aa7e2a37f9625 fromic_a2018m10v9p2370.pdf Title: Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity Subject: Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Keywords: Penicillium expansum, PeAfpA, Penicillium chrysogenum, Penicillium digitatum, Botrytis cinerea, postharvest, crop protection, pathogenic fungi Author: Paloma Manzanares Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed Oct 3 15:44:41 2018 CEST ModDate: Wed Oct 3 16:19:00 2018 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 2370551 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- GRJZAD+MinionPro-Regular Type 1 Custom yes yes no 28 0 TXCBNB+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 29 0 KYIWWR+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 30 0 XGTSZQ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 35 0 LDKFAH+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 36 0 RNLHXU+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 37 0 PWZYAN+MinionPro-It Type 1 Custom yes yes no 120 0 VXWSEY+RBLMI Type 1 Builtin yes yes no 128 0 RITMDE+MTSYN Type 1 Builtin yes yes no 130 0 XUJOMI+MTGU Type 1 Builtin yes yes no 131 0 HGLMXG+MinionPro-Bold Type 1 Custom yes yes no 208 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-02-11 05:28:42 CET RepresentationInformation: fromic_a2018m10v9p2370.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-02-10 13:29:24 CET Size: 2370551 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 929 FreeObjects: 3 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Strains, Media, and Growth Conditions Item: Title: Protein Sequences and Structure Prediction Item: Title: Vector Constructions and P. chrysogenum Transformant Strains Generation Item: Title: Protein Production and Purification Item: Title: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Item: Title: Antibody Generation and Western Blot Item: Title: Antimicrobial Activity Assays Item: Title: Hemolytic Activity Assays Item: Title: Protection Assays Against Fungal Infection Caused by P. digitatum in Citrus Fruits Item: Title: Protection Assays Against Fungal Infection Caused by B. cinerea in Tomato Leaves Item: Title: Results Children: Item: Title: P. expansum Encodes up to Three Distinct AFPs From Different Classes but Only Secretes AFP From Class A Item: Title: Recombinant Production of PeAFPs in P. chrysogenum Item: Title: Immunodetection Confirmed the Absence of PeAfpB and PeAfpC in P. expansum Supernatants Item: Title: PeAFPs Structural Modeling Item: Title: Antimicrobial Activity Assays Item: Title: PeAFPs Showed No Hemolytic Activity Item: Title: PeAfpA Confers Protection Against P. digitatum Infection in Orange Fruits Item: Title: PeAfpA Confers Protection Against B. cinerea Infection in Tomato Leaves Item: Title: Discussion Item: Title: Conclusion Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Three Antifungal Proteins From Penicillium expansum: Different Patterns of Production and Antifungal Activity Author: Paloma Manzanares Subject: Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. 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4e57800de46c444d0b89af2a172128ee Type: MD5 Checksum: 77c03601ed56f752d26dddc4404fab6520b22a43 Type: SHA-1