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Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Background Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Children: Item: Title: Cats and sampling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Diagnosis of Leishmania infection based on cytology, histopathology and immunohistochemistry of cutaneous lesions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Detection of antibodies against L. infantum and L. braziliensis antigens by quantitative enzyme-linked immunosorbent assay (ELISA) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Western blot (WB) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: DNA extraction from blood, paraffin-embedded skin biopsies and cytology from skin lesions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Children: Item: Title: Blood DNA purification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Purification of genomic DNA from formalin-fixed, paraffin-embedded skin biopsies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Purification of genomic DNA from cytological slides from skin lesions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: Leishmania spp. kinetoplast quantitative polymerase chain reaction (qPCR) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Internal transcribed spacer 1 (ITS1) restriction fragment length polymorphism (RFLP), quantitative PCR (qPCR), sequencing and phylogenetic analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Children: Item: Title: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of amplified ITS-1 sequences Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Quantitative PCR high-resolution melting (qPCR-HRM) Leishmania genotyping based on a ITS1, sequencing and phylogenetic analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Detection of FeLV antigen and FIV antibody Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Children: Item: Title: Cats Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Cytology, histopathology and immunohistochemistry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: ELISA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: Western blot Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: Blood and cutaneous lesions Leishmania kinetoplast qPCR, ITS1 RFLP and qPCR for identification, sequencing and phylogenetic analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: Detection of FeLV antigen and FIV antibodies and relationship with Leishmania infection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: Abbreviations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: Availability of data and materials Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: Authors’ contributions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: Ethics approval and consent to participate Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: Consent for publication Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: Competing interests Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: Publisher’s Note Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: Author details Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Info: Title: Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela Author: Aruanai Kalú Rivas Subject: Parasites & Vectors, 2018, doi:10.1186/s13071-018-2747-2 Keywords: Leishmania mexicana,Leishmania infantum,Leishmania braziliensis,Cats,Nodular-ulcerative lesions,ELISA,qPCR,Immunohistochemistry,Venezuela Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.1.5 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Sat Mar 03 06:31:02 CET 2018 ModDate: Mon Mar 05 18:27:51 CET 2018 ID: 0x2983eff68376fa95813655cee4374433, 0x25c307a18e222871974c1011a68e45b4 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 95 ImageHeight: 91 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 688 ImageHeight: 126 BitsPerSample: 8 BitsPerSampleUnit: integer 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