03c142ddef574621fd9154f0e297ff95 pmc_29599471.pdf 247bbf336bdef212d9b4eff94a73a94e1ef342c0 pmc_29599471.pdf 5ab645718a285b12cb8941e024a42e1f733b71ef66d9b3244a3566628ef18927 pmc_29599471.pdf Title: Activation of Eosinophils and Mast Cells in Functional Dyspepsia: an Ultrastructural Evaluation Subject: Scientific Reports, doi:10.1038/s41598-018-23620-y Keywords: Author: Hanne Vanheel Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Mar 22 21:19:25 2018 CET ModDate: Fri Mar 23 12:53:38 2018 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 1708298 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JFPGYL+Corbel CID TrueType Identity-H yes yes yes 348 0 XNWYMJ+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 349 0 MinionPro-Regular Type 1C Custom yes no yes 33 0 YFDSAO+Corbel-Bold2 Type 1C WinAnsi yes yes yes 30 0 WDYNJI+Corbel2 Type 1C WinAnsi yes yes yes 32 0 GOXJIR+Corbel-Bold CID TrueType Identity-H yes yes yes 53 0 EuclidSymbol-Bold Type 1C Custom yes no yes 350 0 YFDSAO+Corbel-Bold2 Type 1C WinAnsi yes yes yes 260 0 WDYNJI+Corbel2 Type 1C WinAnsi yes yes yes 276 0 JFPGYL+Corbel CID TrueType Identity-H yes yes yes 385 0 MyriadPro-It Type 1C WinAnsi yes no yes 261 0 MinionPro-It Type 1C Custom yes no yes 29 0 EuclidSymbol Type 1C Custom yes no yes 31 0 WDYNJI+Corbel2 Type 1C WinAnsi yes yes yes 251 0 JFPGYL+Corbel CID TrueType Identity-H yes yes yes 246 0 EuclidSymbol-Italic Type 1C Custom yes no yes 50 0 LNAVAH+STIXGeneral-Regular Type 1C Custom yes yes yes 51 0 MinionPro-Bold Type 1C WinAnsi yes no yes 52 0 WDYNJI+Corbel2 Type 1C WinAnsi yes yes yes 273 0 JFPGYL+Corbel CID TrueType Identity-H yes yes yes 272 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-06-11 02:27:04 CEST RepresentationInformation: pmc_29599471.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2018-04-09 03:49:53 CEST Size: 1708298 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 4. Vanheel, H. & Farre, R. Changes in gastrointestinal tract function and structure in functional dyspepsia. Nat Rev Gastroenterol Hepatol 10, 142–149, https://doi.org/10.1038/nrgastro.2012.255 (2013). :28' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 4. Vanheel, H. & Farre, R. Changes in gastrointestinal tract function and structure in functional dyspepsia. Nat Rev Gastroenterol Hepatol 10, 142–149, https://doi.org/10.1038/nrgastro.2012.255 (2013). :29' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 3. Talley, N. J. et al. Non-ulcer dyspepsia and duodenal eosinophilia: an adult endoscopic population-based case-control study. Clin Gastroenterol Hepatol 5, 1175–1183, https://doi.org/10.1016/j.cgh.2007.05.015 (2007). :31' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 5. Du, L. et al. Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study. Sci Rep 6, 34305, https://doi.org/10.1038/srep34305 (2016). :41' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 7. Friesen, C. A., Andre, L., Garola, R., Hodge, C. & Roberts, C. Activated duodenal mucosal eosinophils in children with dyspepsia: a pilot transmission electron microscopic study. J Pediatr Gastroenterol Nutr 35, 329–333 (2002). :42' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 8. Vanheel, H. et al. Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia. Gut 63, 262–271, https://doi.org/10.1136/gutjnl-2012-303857 (2014). :43' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 1. Evaluation of low-grade inflammation. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were stained for identification of eosinophils using anti-human MBP (A,B) and mast cells using anti-human tryptase:45' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 1. Evaluation of low-grade inflammation. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were stained for identification of eosinophils using anti-human MBP (A,B) and mast cells using anti-human tryptas:46' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 2. Ultrastructural evaluation of mucosal eosinophils. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were used to evaluate degranulation of eosinophils using transmission electron microscopy. Visual analys:47' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 3. Comparison of visual and quantitative analysis of eosinophilic degranulation. (A) The mean intensity per granule was compared between the three groups: no (n⁜琀㴠㄀㌀Ⰰ 氀漀眀 渠㴠㄀㤀 愀渀搀 栀椀最栀 渠㴠㠀 搀攀最爀愀渀甀氀愀琀椀漀渀⸀ ＀䋾＀ 䌀漀爀爀攀氀愀琀椀漀渀 戀攀琀眀攀㨀㔀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈱㤮㠶㘠㘰㐮㜶㌠㈳〮㐰㔠㔹㈮㘲㉝⽈⽎⽁值㰯丠㠱‰⁒㸾㸾੥湤潢樊㘵‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_23620.indd:Figure 3. Comparison of visual and quantitative analysis of eosinophilic degranulation. (A) The mean intensity per granule was compared between the three groups: no (n⁜琀㴠㄀㌀Ⰰ 氀漀眀 渠㴠㄀㤀 愀渀搀 栀椀最栀 渠㴠㠀 搀攀最爀愀渀甀氀愀琀椀漀渀⸀ ＀䋾＀ 䌀漀爀爀攀氀愀琀椀漀渀 戀攀琀眀㨀㔀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㔮㠸㐠㔵㐮㜶㌠㈴㠮㘶‵㐲⸶㈲崯䠯丯䅐㰼⽎‸㈠〠刾㸾㸊敮摯扪ਸ਼㘠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㈀㌀㘀㈀　⸀椀渀搀搀㫾￾￾＀䘀椀最甀爀攀 㓾￾＀⻾￾＠˾￾￾＀唀氀琀爀愀猀琀爀甀挀琀甀爀愀氀 攀瘀愀氀甀愀琀椀漀渀 漀昀 洀甀挀漀猀愀氀 洀愀猀琀 挀攀氀氀猀⸀ 䐀甀漀搀攀渀愀氀 戀椀漀瀀猀礀 猀愀洀瀀氀攀猀 昀爀漀洀 栀攀愀氀琀栀礀 瘀漀氀甀渀琀攀攀爀猀 挀漀渀琀爀漀氀 愀渀搀 瀀愀琀椀攀渀琀猀 眀椀琀栀 䘀䐀 眀攀爀攀 甀猀攀搀 琀漀 攀瘀愀氀甀愀琀攀 搀攀最爀愀渀甀氀愀琀椀漀渀 漀昀 洀愀猀琀 挀攀氀氀猀 甀猀椀渀最 琀爀愀渀猀洀椀猀猀椀漀渀 攀氀攀挀琀爀漀渀 洀椀挀爀漀猀挀漀瀀礀⸀ 䐀攀最爀攀攀 漀昀 搀攀最爀愀渀㨀㔀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔳㈮㤴㠠㔲㐮㜶㌠㔵ㄮ㔲㐠㔱㈮㘲㉝⽈⽎⽁值㰯丠㠳‰⁒㸾㸾੥湤潢樊㘷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_23620.indd:Figure 4. Ultrastructural evaluation of mucosal mast cells. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were used to evaluate degranulation of mast cells using transmission electron microscopy. Degree of degra:54' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 3. Comparison of visual and quantitative analysis of eosinophilic degranulation. (A) The mean intensity per granule was compared between the three groups: no (n⁜琀㴠㄀㌀Ⰰ 氀漀眀 渠㴠㄀㤀 愀渀搀 栀椀最栀 渠㴠㠀 搀攀最爀愀渀甀氀愀琀椀漀渀⸀ ＀䋾＀ 䌀漀爀爀攀氀愀琀椀漀渀 戀攀琀眀㨀㔀㈩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㔮㠸㐠㔵㐮㜶㌠㈴㠮㘶‵㐲⸶㈲崯䠯丯䅐㰼⽎‸㈠〠刾㸾㸊敮摯扪ਸ਼㘠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㈀㌀㘀㈀　⸀椀渀搀搀㫾￾￾＀䘀椀最甀爀攀 㓾￾＀⻾￾＠˾￾￾＀唀氀琀爀愀猀琀爀甀挀琀甀爀愀氀 攀瘀愀氀甀愀琀椀漀渀 漀昀 洀甀挀漀猀愀氀 洀愀猀琀 挀攀氀氀猀⸀ 䐀甀漀搀攀渀愀氀 戀椀漀瀀猀礀 猀愀洀瀀氀攀猀 昀爀漀洀 栀攀愀氀琀栀礀 瘀漀氀甀渀琀攀攀爀猀 挀漀渀琀爀漀氀 愀渀搀 瀀愀琀椀攀渀琀猀 眀椀琀栀 䘀䐀 眀攀爀攀 甀猀攀搀 琀漀 攀瘀愀氀甀愀琀攀 搀攀最爀愀渀甀氀愀琀椀漀渀 漀昀 洀愀猀琀 挀攀氀氀猀 甀猀椀渀最 琀爀愀渀猀洀椀猀猀椀漀渀 攀氀攀挀琀爀漀渀 洀椀挀爀漀猀挀漀瀀礀⸀ 䐀攀最爀攀攀 漀昀 搀攀最爀愀渀㨀㔀㌩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔳㈮㤴㠠㔲㐮㜶㌠㔵ㄮ㔲㐠㔱㈮㘲㉝⽈⽎⽁值㰯丠㠳‰⁒㸾㸾੥湤潢樊㘷‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_23620.indd:Figure 4. Ultrastructural evaluation of mucosal mast cells. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were used to evaluate degranulation of mast cells using transmission electron microscopy. Degree of degra:54' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd:Figure 4. Ultrastructural evaluation of mucosal mast cells. Duodenal biopsy samples from healthy volunteers (control) and patients with FD were used to evaluate degranulation of mast cells using transmission electron microscopy. Degree of degran:53' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 8. Vanheel, H. et al. Impaired duodenal mucosal integrity and low-grade inflammation in functional dyspepsia. Gut 63, 262–271, https://doi.org/10.1136/gutjnl-2012-303857 (2014). :55' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 3. Talley, N. J. et al. Non-ulcer dyspepsia and duodenal eosinophilia: an adult endoscopic population-based case-control study. Clin Gastroenterol Hepatol 5, 1175–1183, https://doi.org/10.1016/j.cgh.2007.05.015 (2007). :61' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 5. Du, L. et al. Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study. Sci Rep 6, 34305, https://doi.org/10.1038/srep34305 (2016). :67' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 7. Friesen, C. A., Andre, L., Garola, R., Hodge, C. & Roberts, C. Activated duodenal mucosal eosinophils in children with dyspepsia: a pilot transmission electron microscopic study. J Pediatr Gastroenterol Nutr 35, 329–333 (2002). :68' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 9. Furuta, G. T. et al. Eosinophils alter colonic epithelial barrier function: role for major basic protein. Am J Physiol Gastrointest Liver Physiol 289, G890–897, https://doi.org/10.1152/ajpgi.00015.2005 (2005). :71' cannot be found Offset: 1312776 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfMalformedException: Invalid name tree Offset: 1312433 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_23620.indd: 20. Dvorak, A. M. Ultrastructural studies of human basophils and mast cells. J Histochem Cytochem 53, 1043–1070, https://doi.org/10.1369/jhc.5R6647.2005 (2005). :77' cannot be found Offset: 1312776 MIMEtype: application/pdf PDFMetadata: Objects: 427 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Activation of Eosinophils and Mast Cells in Functional Dyspepsia: an Ultrastructural Evaluation Children: Item: Title: Material and Methods Children: Item: Title: Participants. Item: Title: Duodenal biopsies. Children: Item: Title: Immunohistochemistry. Item: Title: Transmission electron microscopy. Item: Title: Ussing chamber experiments. Item: Title: Statistical analysis. Item: Title: Results Children: Item: Title: Study population. Item: Title: Patients with functional dyspepsia are characterized by an increased number of eosinophils and mast cells. Item: Title: Ultrastructural changes in degranulation of eosinophils and mast cells in functional dyspepsia. Item: Title: No association of duodenal epithelial integrity with eosinophils and mast cells. Item: Title: Discussion Item: Title: Acknowledgements Item: Title: Figure 1 Evaluation of low-grade inflammation. Item: Title: Figure 2 Ultrastructural evaluation of mucosal eosinophils. Item: Title: Figure 3 Comparison of visual and quantitative analysis of eosinophilic degranulation. Item: Title: Figure 4 Ultrastructural evaluation of mucosal mast cells. Info: Title: Activation of Eosinophils and Mast Cells in Functional Dyspepsia: an Ultrastructural Evaluation Author: Hanne Vanheel Subject: Scientific Reports, doi:10.1038/s41598-018-23620-y Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Mar 22 21:49:25 CET 2018 ModDate: Fri Mar 23 12:53:38 CET 2018 ID: 0x979608025917bd66c234c1b736e2cd34, 0x9546aee868908003f4a92181f987daf8 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1500 ImageHeight: 1066 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1500 ImageHeight: 2263 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1350 ImageHeight: 1393 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1350 ImageHeight: 793 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: GOXJIR+Corbel-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: JFPGYL+Corbel Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: WDYNJI+Corbel2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: WDYNJI+Corbel2 Flags: Nonsymbolic FontBBox: -553, -239, 1110, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: EuclidSymbol-Italic FirstChar: 0 LastChar: 255 FontDescriptor: FontName: EuclidSymbol-Italic Flags: Symbolic, Italic FontBBox: -166, -276, 1162, 950 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: LNAVAH+STIXGeneral-Regular FontSubset: true FirstChar: 29 LastChar: 31 FontDescriptor: FontName: LNAVAH+STIXGeneral-Regular Flags: Serif, Symbolic FontBBox: -995, -455, 2000, 1055 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: XNWYMJ+TimesNewRomanPSMT FontSubset: true FirstChar: 32 LastChar: 120 FontDescriptor: FontName: XNWYMJ+TimesNewRomanPSMT Flags: Serif, Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: YFDSAO+Corbel-Bold2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: YFDSAO+Corbel-Bold2 Flags: Nonsymbolic FontBBox: -556, -239, 1155, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: EuclidSymbol-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: EuclidSymbol-Bold Flags: Symbolic FontBBox: -186, -308, 1101, 960 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: EuclidSymbol FirstChar: 0 LastChar: 255 FontDescriptor: FontName: EuclidSymbol Flags: Symbolic FontBBox: -166, -253, 1090, 950 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: JFPGYL+Corbel CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: JFPGYL+Corbel Flags: Symbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Font: BaseFont: GOXJIR+Corbel-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: GOXJIR+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true XMP: application/pdf doi:10.1038/s41598-018-23620-y Springer US Scientific Reports, doi:10.1038/s41598-018-23620-y Activation of Eosinophils and Mast Cells in Functional Dyspepsia: an Ultrastructural Evaluation Hanne Vanheel Maria Vicario Werend Boesmans Tim Vanuytsel Eloisa Salvo-Romero Jan Tack Ricard Farramp#x000E9; 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